We are trying to get activity out of this enzyme (thiamine pyrophosphokinase) and are having difficulty. Do you guys have any recommendations that may help us out?

Name: Recombinant Human TPK1 His Protein
Brand: Novus Biologicals
SKU: NBP1-99102

I am sorry for any confusion regarding our protein product NBP1-99102. This antibody is sold with a reducing agent present in the buffer as the protein is advertised for use in SDS PAGE gels as a positive control. Our lab has not yet tested this protein for use in functional assays that require activity.

Recombinant Human TPK1 His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-99102

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Product Datasheet / COA

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all TPK1 Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-243 of Human TPK1

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMEHAFT PLEPLLSTGN LKYCLVILNQ PLDNYFRHLW NKALLRACAD GGANRLYDIT EGERESFLPE FINGDFDSIR PEVREYYATK GCELISTPDQ DHTDFTKCLK MLQKKIEEKD LKVDVIVTLG GLAGRFDQIM ASVNTLFQAT HITPFPIIII QEESLIYLLQ PGKHRLHVDT GMEGDWCGLI PVGQPCMQVT TTGLKWNLTN DVLAFGTLVS TSNTYDGSGV VTVETDHPLL WTMAIKS

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

29.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human TPK1 His Protein

SDS-PAGE: Recombinant Human TPK1 His Protein [NBP1-99102]

SDS-Page: Recombinant Human TPK1 Protein [NBP1-99102] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain

Formulation, Preparation and Storage

NBP1-99102

Formulation	20 mM Tris-HCl buffer (pH8.0), 20% glycerol, 1 mM DTT
Preservative	No Preservative
Concentration	1 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TPK1

TPK1, also known as thiamin pyrophosphokinase 1, catalyzes the phosphorylation of thiamine to thiamine pyrophosphate. This protein can also catalyze the phosphorylation of pyrithiamine to pyrithiamine pyrophosphate. Recombinant human TPK1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Alternate Names

EC 2.7.6.2, HTPK1, Placental protein 20, PP20thiamine kinase, thiamin pyrophosphokinase 1, thiamine diphosphokinase, Thiamine pyrophosphokinase 1

Gene Symbol

TPK1

Additional TPK1 Products

Product Documents for Recombinant Human TPK1 His Protein

Product Datasheets

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COA

Product Specific Notices for Recombinant Human TPK1 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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