Recombinant Mouse TRANCE/TNFSF11/RANK L Animal-Free Protein

Name: Recombinant Mouse TRANCE/TNFSF11/RANK L Animal-Free Protein
Brand: Novus Biologicals
SKU: NBP2-76186

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-76186

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all TRANCE/TNFSF11/RANK L Proteins and Enzymes »

Product Specifications

Description

An un-tagged bioactive recombinant protein corresponding to Mouse TRANCE/TNFSF11/RANK L

Source: E. coli

Amino Acid Sequence: MPAMMEGSWLD VAQRGKPEAQ PFAHLTINAA SIPSGSHKVT LSSWYHDRGW AKISNMTLSN GKLRVNQDGF YYLYANICFR HHETSGSVPT DYLQLMVYVV KTSIKIPSSH NLMKGGSTKN WSGNSEFHFY SINVGGFFKL RAGEEISIQV SNPSLLDPDQ DATYFGAFKV QDID

Produced with no materials of animal or human origin using ANIMAL-FREE purification processes.

Production and Purification

No animal-derived ingredients, No FBS
E. coli based production system
Animal-Free facility, fermentor, columns, filters, labware, lyophilization, and storage

Quality Assurance

Endotoxin levels at <1 EUs/ug
Purity levels at 95% or greater

Purity

>95%, by SDS-PAGE

Endotoxin Level

Acceptance criteria for endotoxin levels are <1 EUs/ug protein (determined by Kinetic LAL Method).

Predicted Molecular Mass

19.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Activity of RAW-BlueTM cells, >2 x 10^4 units/mg (ED50 acceptance criteria are <50 ng/mL).

Specificity

TRANCE/TNFSF11/RANK L is a monomer, 19.5 kDa (175 aa)

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Mouse TRANCE/TNFSF11/RANK L Animal-Free Protein

SDS-PAGE: Recombinant Mouse TRANCE/TNFSF11/RANK L Animal-Free Protein [NBP2-76186]

SDS-Page: Recombinant Mouse TRANCE/TNFSF11/RANK L Animal-Free Protein [NBP2-76186] - 1 ug in each lane (-) non-reducing conditions and (+) reducing conditions in a 4-20% Tris-Glycine gel.

Bioactivity: Recombinant Mouse TRANCE/TNFSF11/RANK L Animal-Free Protein [NBP2-76186] - TRANCE/TNFSF11/RANK L Induced Production of TNF alpha from RAW cells.

Formulation, Preparation and Storage

NBP2-76186

Preparation Method	Protein is derived from genetically modified E. coli.
Formulation	Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate and 20 mM sodium chloride, pH 7.5
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Sterile water at 0.1 mg/mL
Format	Animal Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: TRANCE/TNFSF11/RANK L

TRANCE/OPGL/RANKL/ODF is a recently identified member of tumor necrosis factor family. TRANCE (TNF-related activation-induced cytokine receptor) or RANKL {receptor activator of NF-kB (RANK) ligand} has been implicated in interactions between T cells and dendritic cells (1-2). RANK ligand (RANKL/TRANCE) binds to RANK on dendritic cells, upregulates the expression of anti-apoptotic protein BcL-XL suggesting a role in dendritic cell survival (2). TRANCE/RANKL is also important in T and B-cell maturation (3). As OPGL/ODF (Osteoprotegerin Ligand/Osteoclast differentiation factor), the same protein can both activate mature osteoclasts and mediate osteoclastogenesis (4). OPGL/TRANCE deficient mice show severe osteoporesis and complete absence of osteoclasts as a result of lack of osteogenesis (3).

Long Name

TNF-related Activation-induced Cytokine

Alternate Names

CD254, ODF, OPGL, RANK L, RANKL, TNFSF11

Gene Symbol

TNFSF11

Additional TRANCE/TNFSF11/RANK L Products

Product Documents for Recombinant Mouse TRANCE/TNFSF11/RANK L Animal-Free Protein

Product Datasheets

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COA

Product Specific Notices for Recombinant Mouse TRANCE/TNFSF11/RANK L Animal-Free Protein

Lot Specific Information:
Given endotoxin and ED50 values are acceptance criteria standards. For lot specific values, please contact technical support.

Detailed Reconstitution:
Centrifuge vial before opening. Suspend the product by gently pipetting the recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots.

Expiration Date:
12 months from date of receipt when stored at -20C to-80C as supplied. 1 month when stored at 4C after reconstituting as directed. 3 months when stored at -20C to -80C after reconstituting as directed.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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