Transferrin Native Protein
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-97069
Key Product Details
Product Specifications
Description
Native Human Transferrin
Source: Normal serum (Accession #:Q06AH7)
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
No test method can provide total assurance that the hepatitis B virus, hepatitis C virus, human immunodeficiency virus, or any other infectious agents are absent. Thus, all blood products, including purified proteins derived from human blood sources, should be handled at Biosafety Level 2 as recommended by the CDC\NIH manual entitled Biosafety in Microbiological and Biomedical Laboratories for potentially infectious human serum, blood specimens or proteins derived from same. Source material for the human blood product supplied to your facility has been tested for the detection of HIV antibody, Hepatitis B surface antigen, antibody to Hepatitis C, HIV 1 antigen(s), antibody to HTLV - I/II, and syphilis by FDA guidelines. All units were found to be non-reactive/negative for these tests. All human blood source material is collected in FDA licensed centers and is tested with FDA approved test kits.
Source: Normal serum (Accession #:Q06AH7)
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
No test method can provide total assurance that the hepatitis B virus, hepatitis C virus, human immunodeficiency virus, or any other infectious agents are absent. Thus, all blood products, including purified proteins derived from human blood sources, should be handled at Biosafety Level 2 as recommended by the CDC\NIH manual entitled Biosafety in Microbiological and Biomedical Laboratories for potentially infectious human serum, blood specimens or proteins derived from same. Source material for the human blood product supplied to your facility has been tested for the detection of HIV antibody, Hepatitis B surface antigen, antibody to Hepatitis C, HIV 1 antigen(s), antibody to HTLV - I/II, and syphilis by FDA guidelines. All units were found to be non-reactive/negative for these tests. All human blood source material is collected in FDA licensed centers and is tested with FDA approved test kits.
Purity
Multi-step
Applications
Dot Blot (1:100 - 1:2000)
ELISA (1:4000 - 1:10000)
Immunohistochemistry (1:200 - 1:2000)
Western Blot (1:2000 - 1:5000)
ELISA (1:4000 - 1:10000)
Immunohistochemistry (1:200 - 1:2000)
Western Blot (1:2000 - 1:5000)
Application Notes
This product has been tested in dot blot and is a suitable protein for use as a control reagent in both Western Blotting and ELISA experiments.
NOTE: Do NOT add Sodium Azide (it inhibits HRP irreversibly).
NOTE: Do NOT add Sodium Azide (it inhibits HRP irreversibly).
Protein / Peptide Type
Native Protein
Scientific Data Images for Transferrin Native Protein
Dot Blot: Transferrin Native Protein [NBP1-97069] - Dot Blot result of Human Transferrin Peroxidase conjugate. Dots are Human Transferrin HRP at (1) 100 ng, (2) 33.3 ng, (3) 11.1 ng, (4) 3.70 ng, (5) 1.23 ng. Block for 60 min at RT. Primary Antibody: none. Secondary Antibody: none. Imaged with BioRad ChemiDoc, Chemi filter.
Transferrin Native Protein
Dot Blot result of Transferrin Native Protein. Dots are Human Transferrin HRP at (1) 100ng, (2) 33.3ng, (3) 11.1ng, (4) 3.70ng, (5) 1.23ng. blocking for 60 min at RT. Primary Antibody: none. Secondary Antibody: none. Imaged with BioRad ChemiDoc, Chemi filter.Formulation, Preparation and Storage
NBP1-97069
| Preparation Method | This product was prepared from normal serum by a multi-step process including selective precipitation and tandem chromatography followed by extensive dialysis against the buffer stated above. Human Transferrin Peroxidase conjugated was assayed by immunoelectrophoresis and resulted in a single precipitin arc against anti-Peroxidase, anti-Human Transferrin and anti-Human Serum. |
| Formulation | Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/ml Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free |
| Preservative | 0.01% Gentamicin Sulfate |
| Concentration | Lyoph |
| Reconstitution | Reconstitute with 1.0 ml deionized water. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles. |
Background: Transferrin
Alternate Names
Beta-1 metal-binding globulin, Serotransferrin, Siderophilin, TF
Gene Symbol
TF
UniProt
Additional Transferrin Products
Product Specific Notices for Transferrin Native Protein
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.
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