Recombinant Human TWEAK R/TNFRSF12 Protein

Name: Recombinant Human TWEAK R/TNFRSF12 Protein
Brand: Novus Biologicals
SKU: NBP2-35085

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35085

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Product Datasheet / COA

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Accession #

Q9NP84

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all TWEAK R/TNFRSF12 Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 53 amino acids corresponding to TWEAK R/TNFRSF12 Source: E. coli

Uniprot ID: Q9NP84

Amino Acid Sequence: EQAPGTAPCS RGSSWSADLD KCMDCASCRA RPHSDFCLGC AAAPPAPFRL LWP

Purity

>95%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of TWEAK R/TNFRSF12 as determined by LAL method.

Predicted Molecular Mass

5.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

TWEAK R/TNFRSF12 protein is fully biologically active when compared to standard. The ED50 as determined by inhibiting TWEAK-dependent proliferation of human umbilical vein endothelial cells (HUVEC) is less than 30 ng/ml, corresponding to a specific activity of > 3.3 x104 IU/mg, in the presence of 15 ng/ml of rHuTWEAK

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human TWEAK R/TNFRSF12 Protein

SDS-PAGE: Recombinant Human TWEAK R/TNFRSF12 Protein [NBP2-35085]

SDS-Page: Recombinant Human TWEAK R/TNFRSF12 Protein [NBP2-35085]

Formulation, Preparation and Storage

NBP2-35085

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: TWEAK R/TNFRSF12

Human TNF-related weak inducer of apoptosis receptor (TWEAKR) also known as Tumor necrosis factor receptor superfamily member 12A precursor (gene name TNFRSF12A) or fibroblast growth factor-inducable 14 kDa protein, is distantly related to the TNFR family, containing one cysteine-rich domain in the extracellular region and a TNFR-associated factor binding domain but does not contain a death domain (DD) cytoplasmic region. It is expressed in the spleen, thymus, peripheral blood lymphocytes, colon, and small intestine. Signal transduction by TWEAK receptor can be activated by either the membrane anchored or the soluble TWEAK. In addition, It plays a role in TWEAK-induced endothelial cell migration, proliferation, and angiogenesis. Human and mouse TWEAK R share 82 % a.a. sequence identity

Long Name

TNF-like and Weak Inducer of Apoptosis Receptor

Alternate Names

CD266, Fn14, TNFRSF12, TNFRSF12A, TWEAKR, TWEAK Receptor/TNFRSF12A

Gene Symbol

TNFRSF12A

UniProt