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TWEAK/TNFSF12 Recombinant Protein Antigen

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-46811PEP

Novus Biologicals, part of Bio-Techne
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NBP2-46811PEP

Key Product Details

Source

E. coli

Conjugate

Unconjugated

Applications

Antibody Competition

Product Specifications

Description

A recombinant protein antigen with a N-terminal His6-ABP tag corresponding to human TNFSF12.

Source: E. coli

Amino Acid Sequence: QEPAQEELVAEEDQDPSELNPQTEESQDPAPFLNRLVRPRRSAPKGRKTRARRAIAAHYEVHPRPGQDGAQAGVDGTVSGWEEARINSSSPLRYNRQIGEFIVTRAGLYYLYC

Fusion Tag: N-terminal His6ABP (ABP = Albumin Binding Protein derived from Streptococcal Protein G)

This product is intended to be used as a blocking antigen for antibody competition assays. Any other use of this antigen is done at the risk of the user. The use of this product for commercial production is strictly prohibited. Please contact technical support if you have any questions.

Purity

>80% by SDS-PAGE and Coomassie blue staining

Predicted Molecular Mass

30 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Applications

Antibody Competition (10-100 molar excess)

Application Notes

This recombinant antigen is only intended to be used as a blocking agent to confirm antibody specificity with the corresponding antibody, catalog number NBP2-46811.

It is purified by IMAC chromatography, and the expected concentration is greater than 0.5 mg/ml.

For current lot information, including availability, please contact our technical support team click nb-technical@bio-techne.com

Protein / Peptide Type

Recombinant Protein Antigen

Formulation, Preparation and Storage

NBP2-46811PEP
Formulation PBS and 1M Urea, pH 7.4.
Preservative No Preservative
Concentration Please see the vial label for concentration. If unlisted please contact technical services.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -20C. Avoid freeze-thaw cycles.

Background: TWEAK/TNFSF12

CD266 is a 14 kD transmembrane protein known as TWEAK receptor (TWEAK-R) or fibroblast growth factor inducible 14 (Fn14). It is member 12A of the tumor necrosis factor receptor superfamily (TNFRSF12A) expressed at low levels in a variety of tissues, such as heart, placenta, lung, muscle, and pancreas. TWEAK-R binds TWEAK (CD255) and interacts with the cytoplasmic proteins TRAF1, 2, and 3. The engagement of Fn14 can induce cell death and may play a role in inflammation, hepatocyte growth, and liver neoplasia. ITEM-4 antibody reacts with both mouse and human Fn14. It is useful for flow cytometric analysis.

Long Name

TNF-like and Weak Inducer of Apoptosis

Alternate Names

TNFSF12

Gene Symbol

TNFSF12

Additional TWEAK/TNFSF12 Products

Product Documents for TWEAK/TNFSF12 Recombinant Protein Antigen

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for TWEAK/TNFSF12 Recombinant Protein Antigen

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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