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Recombinant Human UBE2K/E2-25K His (N-Term) Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35096

Novus Biologicals, part of Bio-Techne
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NBP2-35096-100ug
NBP2-35096-10ug
NBP2-35096-1mg
NBP2-35096-500ug

Key Product Details

Source

E. coli

Accession #

Tag

His (N-Term)

Conjugate

Unconjugated

Applications

SDS-PAGE

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 200 amino acids corresponding to UBE2K/E2-25K and 8 amino acid vector sequence including 6x His tag at N-terminus Source: E. coli

Uniprot ID: P61086

Amino Acid Sequence: MHHHHHHAMA NIAVQRIKRE FKEVLKSEET SKNQIKVDLV DENFTELRGE IAGPPDTPYE GGRYQLEIKI PETYPFNPPK VRFITKIWHP NISSVTGAIC LDILKDQWAA AMTLRTVLLS LQALLAAAEP DDPQDAVVAN QYKQNPEMFK QTARLWAHVY AGAPVSSPEY TKKIENLCAM GFDRNAVIVA LSSKSWDVET ATELLLSN

Purity

>95%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of UBE2K/E2-25K as determined by LAL method.

Predicted Molecular Mass

23.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human UBE2K/E2-25K His (N-Term) Protein

SDS-PAGE: Recombinant Human UBE2K/E2-25K His (N-Term) Protein [NBP2-35096]

SDS-PAGE: Recombinant Human UBE2K/E2-25K His (N-Term) Protein [NBP2-35096]

SDS-Page: Human UBE2K/E2-25K His Protein [NBP2-35096]

Formulation, Preparation and Storage

NBP2-35096
Formulation A 0.2 um filtered concentrated solution in 50 mM HEPES, pH 8.0, with 100 mM NaCl, 10 % Glycerol, 1 mM DTT.
Preservative 5% Trehalose
Concentration Please see the vial label for concentration. If unlisted please contact technical services.
Reconstitution Please contact technical support for detailed reconstitution instructions.
Format Carrier-Free
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -20C. Avoid freeze-thaw cycles.

Background: UBE2K/E2-25K

Ubiquitin-conjugating enzyme E2 K (UBE2K) is a protein that in humans is encoded by the UBE2K gene. The protein encoded by this gene belongs to the ubiquitin-conjugating enzyme family. It binds selectively to a large region at the N terminus of huntingtin. This interaction is not influenced by the length of the huntingtin polyglutamine tract. This protein has been implicated in the degradation of huntingtin and suppression of apoptosis.

Long Name

Ubiquitin-conjugating Enzyme E2

Alternate Names

E2-25K, E225K, Hip2, HYPG, LIG, UbcH1, UBE2K, UBE2K-25K

Gene Symbol

UBE2K

UniProt

Additional UBE2K/E2-25K Products

Product Documents for Recombinant Human UBE2K/E2-25K His (N-Term) Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Human UBE2K/E2-25K His (N-Term) Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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