Recombinant Human UDP glucose dehydrogenase His Protein

Name: Recombinant Human UDP glucose dehydrogenase His Protein
Brand: Novus Biologicals
SKU: NBP1-45276

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-45276

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

Enzyme Activity, SDS-PAGE

View all UDP glucose dehydrogenase Proteins and Enzymes »

Product Specifications

Description

A bioactive recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-494 of Human UDP glucose dehydrogenase

Source: E.coli

Amino Acid Sequence: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSELEM FEIKKICCIG AGYVGGPTCS VIAHMCPEIR VTVVDVNESR INAWNSPTLP IYEPGLKEVV ESCRGKNLFF STNIDDAIKE ADLVFISVNT PTKTYGMGKG RAADLKYIEA CARRIVQNSN GYKIVTEKST VPVRAAESIR RIFDANTKPN LNLQVLSNPE FLAEGTAIKD LKNPDRVLIG GDETPEGQRA VQALCAVYEH WVPREKILTT NTWSSELSKL AANAFLAQRI SSINSISALC EATGADVEEV ATAIGMDQRI GNKFLKASVG FGGSCFQKDV LNLVYLCEAL NLPEVARYWQ QVIDMNDYQR RRFASRIIDS LFNTVTDKKI AILGFAFKKD TGDTRESSSI YISKYLMDEG AHLHIYDPKV PREQIVVDLS HPGVSEDDQV SRLVTISKDP YEACDGAHAV VICTEWDMFK ELDYERIHKK MLKPAFIFDG RRVLDGLHNE LQTIGFQIET IGKKVSSKRI PYAPSGEIPK FSLQDPPNKK PKV

Purity

>95%, by SDS-PAGE

Predicted Molecular Mass

59.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Specific activity is > 2500 pmol/min/ug, and is defined as the amount of enzyme that convert 1.0pmole of UDP-glucose to UDP-glucuronate per minute at pH 8.7 at 37C.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human UDP glucose dehydrogenase His Protein

SDS-PAGE: Recombinant Human UDP glucose dehydrogenase His Protein [NBP1-45276]

SDS-Page: Recombinant Human UDP glucose dehydrogenase Protein [NBP1-45276] - 15% SDS-PAGE (3ug)

Formulation, Preparation and Storage

NBP1-45276

Formulation	20 mM Tris-HCl buffer (pH 8.0), 20% glycerol, 1 mM DTT, 0.1 M NaCl, and 1 mM EDTA.
Preservative	No Preservative
Concentration	0.5 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: UDP glucose dehydrogenase

UGDH is a member of the UDP-glucose/GDP-mannose dehydrogenase family and is a ubiquitously expressed protein most abundant in the liver. This protein converts UDP-glucose to UDP-glucuronate and thereby participates in the biosynthesis of glycosaminoglycans such as hyaluronan, chondroitin sulfate, and heparan sulfate. These glycosylated compounds are common components of the extracellular matrix and likely play roles in signal transduction, cell migration, and cancer growth and metastasis. Recombinant human UGDH protein, fused to his-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques

Alternate Names

EC 1.1.1.22, GDH, UDPGDHUDP-glucose dehydrogenase, UDP-Glc dehydrogenase, UDP-GlcDH, UDP-glucose 6-dehydrogenase, UGD, uridine diphospho-glucose dehydrogenase

Gene Symbol

UGDH

Additional UDP glucose dehydrogenase Products

Product Documents for Recombinant Human UDP glucose dehydrogenase His Protein

Product Datasheets

Download Product Datasheets

Protocols & Manuals

Download SDS-PAGE Protocol

COA

Product Specific Notices for Recombinant Human UDP glucose dehydrogenase His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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