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Recombinant Human UGT8 His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-22928

Novus Biologicals, part of Bio-Techne
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NBP2-22928

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

Product Specifications

Description

A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 21-541 of Human UGT8

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSAKIIIVP PIMFESHMYI FKTLASALHE RGHHTVFLLS EGRDIAPSNH YSLQRYPGIF NSTTSDAFLQ SKMRNIFSGR LTAIELFDIL DHYTKNCDLM VGNHALIQGL KKEKFDLLLV DPNDMCGFVI AHLLGVKYAV FSTGLWYPAE VGAPAPLAYV PEFNSLLTDR MNLLQRMKNT GVYLISRLGV SFLVLPKYER IMQKYNLLPE KSMYDLVHGS SLWMLCTDVA LEFPRPTLPN VVYVGGILTK PASPLPEDLQ RWVNGANEHG FVLVSFGAGV KYLSEDIANK LAGALGRLPQ KVIWRFSGPK PKNLGNNTKL IEWLPQNDLL GHSKIKAFLS HGGLNSIFET MYHGVPVVGI PLFGDHYDTM TRVQAKGMGI LLEWKTVTEK ELYEALVKVI NNPSYRQRAQ KLSEIHKDQP GHPVNRTIYW IDYIIRHNGA HHLRAAVHQI SFCQYFLLDI AFVLLLGAAL LYFLLSWVTK FIYRKIKSLW SRNKHSTVNG HYHNGILNGK YKRNGHIKHE KKVK

Purity

>85%, by SDS-PAGE

Predicted Molecular Mass

61.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Application Notes

Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human UGT8 His Protein

SDS-PAGE: Recombinant Human UGT8 His Protein [NBP2-22928]

SDS-PAGE: Recombinant Human UGT8 His Protein [NBP2-22928]

SDS-Page: UGT8 Protein [NBP2-22928]

Formulation, Preparation and Storage

NBP2-22928
Formulation 20 mM Tris-HCl buffer (pH8.0), 10% glycerol, 0.4M UREA
Preservative No Preservative
Concentration 1 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: UGT8

UDP glycosyltransferase 8, also known as UGT8, catalyzes the transfer of galactose to ceramide, a key enzymatic step in the biosynthesis of galactocerebrosides, which are abundant sphingolipids of the myelin membrane of the central nervous system and peripheral nervous system. Recombinant human UGT8 protein, fused to His-tag at N-terminus, was expressed in E.coli.

Alternate Names

Ceramide UDP-galactosyltransferase, Cerebroside synthase, CGT2-hydroxyacylsphingosine 1-beta-galactosyltransferase, EC 2.4.1, EC 2.4.1.45, UDP glycosyltransferase 8, UDP-galactose ceramide galactosyltransferase, UDP-galactose-ceramide galactosyltransferase, UGT4, uridine diphosphate glycosyltransferase 8

Gene Symbol

UGT8

Additional UGT8 Products

Product Documents for Recombinant Human UGT8 His Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Human UGT8 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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