Goat IgG Horseradish Peroxidase-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # HAF019
Key Product Details
Species Reactivity
Goat
Applications
Immunohistochemistry, Western Blot
Label
HRP
Antibody Source
Polyclonal Chicken IgY
Product Specifications
Immunogen
Goat IgG
Specificity
Detects goat IgG heavy and light chains in direct ELISAs and Western blots. In Western blots, less than 5% cross‑reactivity with mouse IgG, rabbit IgG and human IgG is observed.
Clonality
Polyclonal
Host
Chicken
Isotype
IgY
Scientific Data Images for Goat IgG Horseradish Peroxidase-conjugated Antibody
Detection of Goat IgG Primary Antibody by Western Blot.
Western blot shows detection of Goat Anti-Human/Mouse/Rat Carboxylesterase 1/CES1 Polyclonal Antibody (AF4920) using a 1:1000 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Vimentin in Human Kidney
Detection of Vimentin in human kidney using 5 ug/mL of Goat anti-human Vimentin primary antibody (AF2105) followed by 30 min incubation with Donkey Anti-Goat HRP conjugated secondary antibodies (Catalog# HAF019) at 1:20 dilution + DAB chromogen (brown). The tissue was counterstained with Hematoxylin (blue). Control was done by omitting primary antibody.Applications for Goat IgG Horseradish Peroxidase-conjugated Antibody
Application
Recommended Usage
Immunohistochemistry
Use at a 1:20
dilution
Western Blot
Use at a 1:1000 dilution.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using HAF019 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified from egg yolks
Formulation
Supplied as a 0.2 μm filtered solution in Sodium Phosphate and Proclin 300.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Do not freeze.
- 6 months from date of receipt, 2 to 8 °C as supplied.
Background: IgG
R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.
Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.
Long Name
Immunoglobulin G
Alternate Names
Immunoglobulin G, ImmunoglobulinG
Additional IgG Products
Product Documents for Goat IgG Horseradish Peroxidase-conjugated Antibody
Product Specific Notices for Goat IgG Horseradish Peroxidase-conjugated Antibody
For research use only
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