Goat anti-Mouse IgA/IgG/IgM (H+L) Secondary Antibody (Pre-adsorbed)
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-72561
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Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Mouse
Applications
ELISA, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Format
Pre-adsorbed
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Mouse IgG IgA and IgM whole molecule
Specificity
This antibody was pre-adsorbed against Bovine, Horse, and Human Serum Proteins. No reaction was observed against bovine, horse or human serum proteins. This reagent is suitable for the detection of all mouse isotypes and chain combinations. ELISA was used to confirm specificity at less than 1% of the target signal.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Description
This product was prepared from polyspecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by cross adsorption to remove unwanted specificities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgG, Mouse IgA and Mouse IgM
Store vial at 4C prior to opening. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Store vial at 4C prior to opening. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Applications for Goat anti-Mouse IgA/IgG/IgM (H+L) Secondary Antibody (Pre-adsorbed)
Application
Recommended Usage
ELISA
1:200000
Immunohistochemistry
1:1000 - 1:5000
Western Blot
1:2000 - 1:10000
Application Notes
This product has been tested by ELISA, SDS-PAGE, and Dot Blot, and is suitable for ELISA, western blot, and immunohistochemistry, as well as other assays requiring lot-to-lot consistency.
Formulation, Preparation, and Storage
Purification
Multi-step
Formulation
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Format
Pre-adsorbed
Preservative
0.01% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: IgA/IgG/IgM (H+L)
Additional IgA/IgG/IgM (H+L) Products
Product Documents for Goat anti-Mouse IgA/IgG/IgM (H+L) Secondary Antibody (Pre-adsorbed)
Product Specific Notices for Goat anti-Mouse IgA/IgG/IgM (H+L) Secondary Antibody (Pre-adsorbed)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.
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