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Key Product Details

Species Reactivity

Canine

Applications

Dot Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

1.0 mg/ml

Product Specifications

Immunogen

Dog IgE

Specificity

By immunoelectrophoresis and ELISA this reacts specifically with dog IgE. This may cross react with IgE from other species.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Goat anti-Canine IgE Heavy Chain Secondary Antibody

ELISA: Goat anti-Canine IgE Heavy Chain Secondary Antibody [NB7343]

ELISA: Goat anti-Canine IgE Heavy Chain Secondary Antibody [NB7343]

ELISA: Goat anti-Canine IgE Heavy Chain Secondary Antibody [NB7343] - Sandwich ELISA for total dog IgE measurement showing standard curve at 2 different coating antibody concentrations. Coating: 1ug/ml in carbonate buffer, pH 9.6. Standard: purified dog IgE, range: 156pg/ml to 15.000 pg/ml. Detection with secondary antibody (HRP-labeled) and TMB substrate. This image was submitted via customer Review.
Dot Blot: Goat anti-Canine IgE Secondary Antibody [NB7343] - D.p. extracts have effects on MCs though the IgE-FcepsilonRI axis. SDS-PAGE of recombinants (300 ng) and Western blotting (168.8 ng, for rDer p 15; 143.8 ng of rDer p 18 per gel lane) of specific IgE in canine sera. Image collected and cropped by CiteAb from the following publication (https://waojournal.biomedcentral.com/articles/10.1186/s40413-017-0154-3), licensed under a CC-BY license.. Using the HRP format of this antibody.

Applications for Goat anti-Canine IgE Heavy Chain Secondary Antibody

Application
Recommended Usage

ELISA

1:1000 - 1:30000

Immunocytochemistry/ Immunofluorescence

1:200- 1:2000

Immunohistochemistry

1:200- 1:2000

Immunohistochemistry-Paraffin

1:200 - 1:2000

Western Blot

1:1000 - 1:30000
Application Notes
ELISA 1:1,000 - 1:30,000; for coating plates 1:100 - 1:500

Reviewed Applications

Read 2 reviews rated 5 using NB7343 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Phosphate Buffered Saline (PBS)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: IgE Heavy Chain

Alternate Names

Constant Region of Heavy Chain of IgE, Ig Epsilon Chain C Region, Ig Epsilon Chain C Region ND, IgE, Immunoglobulin Epsilon, immunoglobulin heavy constant epsilon

Additional IgE Heavy Chain Products

Product Documents for Goat anti-Canine IgE Heavy Chain Secondary Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Goat anti-Canine IgE Heavy Chain Secondary Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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