Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunomicroscopy

Label

DyLight 488 (Excitation = 493 nm, Emission = 518 nm)

Antibody Source

Polyclonal Goat IgG

Format

Pre-adsorbed

Concentration

LYOPH mg/ml

View all IgE Heavy Chain Secondary Antibodies »

Product Specifications

Immunogen

Purified human IgE, (Epsilon chain)

Reactivity Notes

Based on IEP, no reactivity is observed to non-immunoglobulin human serum immunoglobulins and light chains on all human immunoglobulins serum proteins from bovine, mouse, or rabbit

Specificity

Based on IEP, this antibody reacts with heavy (epsilon) chains on human IgE

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Description

Purity > 95% based on SDS-PAGE. Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian. After reconstituted, prepare fresh working dilution daily.



Product is stable for up to 4 weeks at 2-8C after rehydration. For extended storage after rehydration, add an equal volume of glycerol and store at -20C.

Applications

Application
Recommended Usage

Flow Cytometry

1:20-1:2000

Immunomicroscopy

1:20-1:2000
Application Notes
This conjugate is suitable for immunomicroscopy and flow cytometry.

Formulation, Preparation, and Storage

Purification

Affinity purified

Reconstitution

Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.

Formulation

10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free

Format

Pre-adsorbed

Preservative

0.05% Sodium Azide

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store lyophilized antibody at 4C. Mix reconstituted liquid with an equal volume of glycerol, aliquot contents and freeze at -20C or below for long term storage.

Background: IgE Heavy Chain

Alternate Names

Constant Region of Heavy Chain of IgE, Ig Epsilon Chain C Region, Ig Epsilon Chain C Region ND, IgE, Immunoglobulin Epsilon, immunoglobulin heavy constant epsilon

Additional IgE Heavy Chain Products

Product Documents

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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