Rabbit anti-Golden Syrian Hamster IgG F(ab')2 Secondary Antibody [Alkaline Phosphatase]
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-72620
Key Product Details
Species Reactivity
Golden Syrian Hamster
Applications
ELISA, Immunohistochemistry, Western Blot
Label
Alkaline Phosphatase
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Hamster IgG F(ab')2 fragment
Specificity
No reaction was observed against Hamster IgG F(c).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Store vial at 4C before opening. DO NOT FREEZE. This product is stable at 4C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Hamster IgG coupled to agarose. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Hamster IgG and Hamster Serum
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Hamster IgG coupled to agarose. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Hamster IgG and Hamster Serum
Applications
Application
Recommended Usage
ELISA
1:2000 - 1:10000
Immunohistochemistry
1:200 - 1:1000
Western Blot
1:500 - 1:2500
Application Notes
This product has been assayed against 1.0 ug of Hamster IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:5,000 of the reconstitution concentration is suggested for this product.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: IgG F(ab')2
Additional IgG F(ab')2 Products
Product Specific Notices
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.
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