Goat anti-Guinea Pig IgG F(ab')2 Secondary Antibody [HRP]
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-73519
Key Product Details
Species Reactivity
Guinea Pig
Applications
ELISA, Immunohistochemistry, Western Blot
Label
HRP
Antibody Source
Polyclonal Goat IgG
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
Guinea Pig IgG F(ab')2 fragment
Specificity
No reaction was observed against Guinea Pig IgG F(c).
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Description
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Guinea Pig IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Guinea Pig IgG, Guinea Pig IgG F(ab')2 and Guinea Pig Serum
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Guinea Pig IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Guinea Pig IgG, Guinea Pig IgG F(ab')2 and Guinea Pig Serum
Applications for Goat anti-Guinea Pig IgG F(ab')2 Secondary Antibody [HRP]
Application
Recommended Usage
ELISA
1:2000 - 1:6000
Immunohistochemistry
1:500 - 1:2500
Western Blot
1:1000 - 1:10000
Application Notes
This product has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lot-to-lot consistency.
NOTE: Do NOT add Sodium Azide (it inhibits HRP irreversibly).
NOTE: Do NOT add Sodium Azide (it inhibits HRP irreversibly).
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Reconstitution
Reconstitute with 1.0 ml deionized water (or equivalent).
Formulation
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% Gentamicin Sulfate
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
Background: IgG F(ab')2
Additional IgG F(ab')2 Products
Product Documents for Goat anti-Guinea Pig IgG F(ab')2 Secondary Antibody [HRP]
Product Specific Notices for Goat anti-Guinea Pig IgG F(ab')2 Secondary Antibody [HRP]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...
Loading...