Key Product Details

Species Reactivity

Human

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # EM-07

Concentration

1 mg/ml

View all IgG Fc Secondary Antibodies »

Product Summary for Mouse anti-Human IgG Fc Secondary Antibody (EM-07)

Immunogen

Fusion protein of human IgG Fc fragment.

Specificity

This secondary antibody reacts with Fc part of human IgG heavy chain and with isolated Fc fragments.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Mouse anti-Human IgG Fc Secondary Antibody (EM-07)

Western Blot: Mouse anti-Human IgG Fc Secondary Antibody (EM-07) [NBP1-45063]

Western Blot: Mouse anti-Human IgG Fc Secondary Antibody (EM-07) [NBP1-45063]

Western Blot: Mouse anti-Human IgG Fc Secondary Antibody (EM-07) [NBP1-45063] - IgG kappa light chain (1), IgG lambda light chain (2) and IgG Fc (3) purified from human serum were analysed by Western blotting with an antibody against IgG kappa light chain (A) and Mouse anti-Human IgG Fc Secondary antibody (EM-07)antibody against IgG Fc fragment (B).
ELISA: Mouse anti-Human IgG Fc Secondary Antibody (EM-07) [NBP1-45063]

ELISA: Mouse anti-Human IgG Fc Secondary Antibody (EM-07) [NBP1-45063]

ELISA: Mouse anti-Human IgG Fc Secondary Antibody (EM-07) [NBP1-45063] - Detection of IgG in human serum using HRP conjugated antibody against IgG Fc fragment.

Applications for Mouse anti-Human IgG Fc Secondary Antibody (EM-07)

Application
Recommended Usage

ELISA

1:2000

Flow Cytometry

1-4 ug/ml

Immunocytochemistry/ Immunofluorescence

1:10-1:2000

Western Blot

1:1000
Application Notes
Western Blot and ELISA: The peroxidase conjugate of this antibody is suitable for detection of human IgG Fc fragments by WB. It does not crossreact with IgM.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Preservative

0.9% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Product Documents for Mouse anti-Human IgG Fc Secondary Antibody (EM-07)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Mouse anti-Human IgG Fc Secondary Antibody (EM-07)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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