Key Product Details

Species Reactivity

Guinea Pig

Applications

Dot Blot, ELISA, Immunohistochemistry, Western Blot

Label

Alkaline Phosphatase

Antibody Source

Polyclonal Goat IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all IgG Fc Secondary Antibodies »

Product Specifications

Immunogen

Guinea Pig IgG F(c) fragment

Specificity

No reaction was observed against Guinea Pig IgG F(ab).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Description

Store vial at 4C before opening. DO NOT FREEZE. This product is stable at 4C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Guinea Pig IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Guinea Pig IgG, Guinea Pig IgG F(c) and Guinea Pig Serum

Scientific Data Images for Goat anti-Guinea Pig IgG Fc Secondary Antibody [Alkaline Phosphatase]

Dot Blot: Goat anti-Guinea Pig IgG Fc Secondary Antibody [Alkaline Phosphatase] [NBP1-72616] - Load: Lane 1 - 200 ng Lane 2 - 66.67 ng Lane 3 - 22.22 ng Lane 4 - 7.41 ng Lane 5 - 2.47 ng. Primary antibody: none. Secondary antibody: Goat anti-Guinea Pig IgG AntibodyAlkaline Phosphatase Conjugated at 1:1,000 for 60 min at RT. Blocked with blocking buffer for 60 min at RT. Visualized using Alkaline Phosphatase Substrate for 30 seconds at RT.
Goat anti-Guinea Pig IgG Fc Secondary Antibody [Alkaline Phosphatase]

Goat anti-Guinea Pig IgG Fc Secondary Antibody [Alkaline Phosphatase]

Dot Blot of Goat anti-Guinea Pig IgG Fc Antibody Alkaline Phosphatase Conjugated. Antigen: Guinea Pig IgG Fc. Load: Lane 1 - 200 ng Lane 2 - 66.67 ng Lane 3 - 22.22 ng Lane 4 - 7.41 ng Lane 5 - 2.47 ng. Primary antibody: none. Secondary antibody: Goat anti-Goat anti-Guinea Pig IgG Fc Secondary Antibody [Alkaline Phosphatase]at 1:1,000 for 60 min at RT. Block for 60 min at RT. Visualized using NBT-100 Alkaline Phosphatase Substrate for 30 seconds at RT.

Applications for Goat anti-Guinea Pig IgG Fc Secondary Antibody [Alkaline Phosphatase]

Application
Recommended Usage

ELISA

1:2000 - 1:10000

Immunohistochemistry

1:200 - 1:1000

Western Blot

1:500 - 1:2500
Application Notes
This product has been tested by ELISA and dot blot. This product has been assayed against 1.0 ug of Guinea Pig IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:5,000 is suggested for this product.

Formulation, Preparation, and Storage

Purification

Multi-step

Formulation

0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Product Documents for Goat anti-Guinea Pig IgG Fc Secondary Antibody [Alkaline Phosphatase]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Goat anti-Guinea Pig IgG Fc Secondary Antibody [Alkaline Phosphatase]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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