Key Product Details

Species Reactivity

Guinea Pig

Applications

Dot Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Alkaline Phosphatase

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all IgG Fc Secondary Antibodies »

Product Specifications

Immunogen

Guinea Pig IgG F(c) fragment

Specificity

No reaction was observed against Guinea Pig IgG F(ab').

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Store vial at 4C before opening. DO NOT FREEZE. This product is stable at 4C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Guinea Pig IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Guinea Pig IgG, Guinea Pig IgG F(c) and Guinea Pig Serum

Scientific Data Images

ELISA: Rabbit anti-Guinea Pig IgG Fc Secondary Antibody [Alkaline Phosphatase] [NBP1-72634] - ELISA Results of Rabbit anti-Guinea Pig IgG Fc Secondary antibody [Alkaline Phosphatase] tested against purified Guinea Pig IgG Fc Alk Phos. Each well was coated in duplicate with 1.0 ug of Guinea Pig IgG Fc. The working dilution is 1:18,000. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Alkaline Phosphatase ELISA Substrate Buffer and NPP Working Buffer.

Applications

Application
Recommended Usage

ELISA

1:5000 - 1:15000

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:1000

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

1:500 - 1:2500
Application Notes
This product has been tested by ELISA and dot blot and is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lot-to-lot consistency.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative

0.1% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Product Documents

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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