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Goat anti-Human IgG Fc Secondary Antibody [FITC]

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-73177

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Dot Blot, Flow Cytometry, Fluorophore-linked immunosorbent assay, Immunocytochemistry/ Immunofluorescence

Label

FITC (Excitation = 495 nm, Emission = 519 nm)

Antibody Source

Polyclonal Goat IgG

Concentration

LYOPH mg/ml

Product Specifications

Immunogen

Human IgG F(c) fragment

Specificity

No reaction was observed against Human IgG F(ab).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Description

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Human IgG, Human IgG F(c) and Human Serum

Scientific Data Images for Goat anti-Human IgG Fc Secondary Antibody [FITC]

Dot Blot: Goat anti-Human IgG Fc Secondary Antibody [FITC] [NBP1-73177] - Lane 1: 100ng. Lane 2: 33.3ng. Lane 3: 11.1ng. Lane 4: 3.7ng. Lane 5: 1.23ng. Secondary Antibody: Gt a-Hu IgG F(c) FITC at 1 ug/mL. Blocking Buffer: BlockOut MB-073 for 30 min at RT.
Goat anti-Human IgG Fc Secondary Antibody [FITC]

Goat anti-Human IgG Fc Secondary Antibody [FITC]

Dot Blot of Goat anti-Human IgG Fc Secondary Antibody [FITC]. Lane 1: 100ng. Lane 2: 33.3ng. Lane 3: 11.1ng. Lane 4: 3.7ng. Lane 5: 1.23ng. Secondary Antibody: Gt a-Hu IgG F(c) FITC at 1ug/mL. Blocking Buffer: BlockOut for 30 min at RT.

Applications for Goat anti-Human IgG Fc Secondary Antibody [FITC]

Application
Recommended Usage

Flow Cytometry

1:500 - 1:2500

Fluorophore-linked immunosorbent assay

1:10000 - 1:50000

Immunocytochemistry/ Immunofluorescence

1:1000 - 1:5000
Application Notes
This product has been tested by dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Formulation, Preparation, and Storage

Purification

Multi-step

Reconstitution

Reconstitute with 1.0 ml deionized water (or equivalent).

Formulation

Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative

0.01% Sodium Azide

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.

Product Documents for Goat anti-Human IgG Fc Secondary Antibody [FITC]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Goat anti-Human IgG Fc Secondary Antibody [FITC]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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