Rabbit anti-Goat IgG Fc Secondary Antibody [HRP] (Pre-adsorbed)
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-74864
Conjugate
Catalog #
Key Product Details
Species Reactivity
Goat
Applications
ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
HRP
Antibody Source
Polyclonal Rabbit IgG
Format
Pre-adsorbed
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
Purified Goat IgG Fc fragment
Reactivity Notes
Based on IEP, no reactivity is observed to non-immunoglobulin goat serum immunoglobulins, light chains on all goat immunoglobulins, goat IgG F(ab)'2 fragment, and human serum proteins
Specificity
Based on IEP, this antibody reacts with heavy gamma chains on goat IgG. This antibody has been pre-adsorbed against human serum proteins.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Purity > 95% based on SDS-PAGE. Rabbit serum was obtained from healthy animals of US origin and under the care of a registered veterinarian. After reconstituted, prepare fresh working dilution daily.
Store lyophilized material at 2-8C. For long term storage after reconstitution, dilute with 50% glycerol and store at -20C as a liquid.
Store lyophilized material at 2-8C. For long term storage after reconstitution, dilute with 50% glycerol and store at -20C as a liquid.
Applications for Rabbit anti-Goat IgG Fc Secondary Antibody [HRP] (Pre-adsorbed)
Application
Recommended Usage
ELISA
1:200-1:5000
Immunohistochemistry
1:500-1:5000
Western Blot
1:200-1:5000
Application Notes
This conjugate is suitable for all immunoassay applications.
Formulation, Preparation, and Storage
Purification
Affinity purified
Reconstitution
Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Formulation
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Format
Pre-adsorbed
Preservative
0.1% Kathon
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store lyophilized antibody at 4C. Mix reconstituted liquid with an equal volume of glycerol, aliquot contents and freeze at -20C or below for long term storage.
Background: IgG Fc
Additional IgG Fc Products
Product Documents for Rabbit anti-Goat IgG Fc Secondary Antibody [HRP] (Pre-adsorbed)
Product Specific Notices for Rabbit anti-Goat IgG Fc Secondary Antibody [HRP] (Pre-adsorbed)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.
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