Goat anti-Human IgG Fc Secondary Antibody [HRP] (Pre-adsorbed)
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-74985
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
HRP
Antibody Source
Polyclonal Goat IgG
Format
Pre-adsorbed
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
Purified Human IgG, Fc fragment
Reactivity Notes
Based on IEP, no reactivity is observed to non-immunoglobulin human serum immunoglobulins, light chains on all human immunoglobulins, human IgG F(ab)'2 fragment, and serum proteins from bovine, horse, mouse, or rabbit
Specificity
Based on IEP, this antibody reacts with heavy gamma chains on human IgG. This antibody has been pre-adsorbed against bovine, horse, mouse, and rabbit serum proteins.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Description
Purity > 95% based on SDS-PAGE. Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian. After reconstituted, prepare fresh working dilution daily.
Store lyophilized material at 2-8C. For long term storage after reconstitution, dilute with 50% glycerol and store at -20C as a liquid.
Store lyophilized material at 2-8C. For long term storage after reconstitution, dilute with 50% glycerol and store at -20C as a liquid.
Applications for Goat anti-Human IgG Fc Secondary Antibody [HRP] (Pre-adsorbed)
Application
Recommended Usage
ELISA
1:200-1:5000
Immunohistochemistry
1:500-1:5000
Western Blot
1:200-1:5000
Application Notes
This conjugate is suitable for all immunoassay applications.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Reconstitution
Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Formulation
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Format
Pre-adsorbed
Preservative
0.1% Kathon
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store lyophilized antibody at 4C. Mix reconstituted liquid with an equal volume of glycerol, aliquot contents and freeze at -20C or below for long term storage.
Background: IgG Fc
UniProt
Additional IgG Fc Products
Product Documents for Goat anti-Human IgG Fc Secondary Antibody [HRP] (Pre-adsorbed)
Product Specific Notices for Goat anti-Human IgG Fc Secondary Antibody [HRP] (Pre-adsorbed)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.
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