Sheep anti-Mouse IgG (H+L) Secondary Antibody [Allophycocyanin]

Novus Biologicals, part of Bio-Techne | Catalog # NB120-6710APC

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Product Datasheet / COA / SDS

View Available Conjugates

Conjugate

Catalog #

Unconjugated

NB120-6710

Alkaline Phosphatase

NB120-6809

Allophycocyanin/Cy7

NB120-6710APCCY7

Biotin

NB120-6807

DyLight 350

NB120-6710UV

DyLight 405

NBP1-72929

DyLight 488

NBP1-72924

DyLight 550

NB120-6710R

DyLight 594

NB120-6710DL594

DyLight 650

NB120-6710C

DyLight 680

NBP1-72927

DyLight 755

NB120-6710IR

DyLight 800

NBP1-72928

FITC

NB120-6804

HRP

NB120-6808

Janelia Fluor 549

NB120-6710JF549

Janelia Fluor 646

NB120-6710JF646

PE

NB120-6710PE

PE/Atto594

NB120-6710PEATT594

PE/Cy5.5

NB120-6710PECY55

PE/Cy7

NB120-6710PECY7

PerCP

NB120-6710PCP

Rhodamine

NB120-6805

Texas Red

NB120-6806

Product Summary
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Mouse

Applications

Dot Blot, ELISA, Fluorophore-linked immunosorbent assay, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Polyclonal Sheep IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all IgG (H+L) Secondary Antibodies »

Product Summary for Sheep anti-Mouse IgG (H+L) Secondary Antibody [Allophycocyanin]

Immunogen

Sheep anti-Mouse IgG (H+L) Secondary Antibody was produced by repeated immunization with Mouse IgG whole molecule in sheep.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Applications for Sheep anti-Mouse IgG (H+L) Secondary Antibody [Allophycocyanin]

Application

Recommended Usage

Dot Blot

Optimal dilutions of this antibody should be experimentally determined.

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Fluorophore-linked immunosorbent assay

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Multi-step

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: IgG (H+L)

Antibodies, also known as immunoglobulins (Igs) are critical for immunity and are grouped into five primary classes: IgG, IgM, IgA, IgD, and IgE. The most abundant antibody isotype is immunoglobulin G (IgG) with concentrations ranging from 7.5-22 mg/ml in human serum and has a molecular weight of 150 kDa. The major effector functions of IgG include neutralization, opsonization, complement fixation and antibody dependent cell-mediated cytotoxicity (ADCC). This monomeric immunoglobulin, expressed on the surface of mature B cells, is often depicted as a Y-shape and comprised of 2 heavy chains and 2 light chains linked by disulfide bonds. The heavy chain is type gamma including subtypes gamma 1, gamma 2, gamma 3, and gamma 4 while the light chain is either a kappa or lambda chain. An IgG molecule has two antigen binding sites, each consisting of a heavy and light chain N-terminal variable domain. When combined with the constant heavy chain 1 (Ch1) and the constant light chain domains, it forms the fragment antigen-binding (Fab) region (2 per antibody). The remaining domains (Ch2-Ch4) of both heavy chains make up the Fc region and contain a site for covalently linking an enzymatic or fluorochrome probe, such as HRP or Janelia Fluor 549, for target detection and visualization (1,2,3).

The 4 IgG subclasses, sharing 95% amino acid identity, include IgG1, IgG2, IgG3, and IgG4 for humans and IgG1, IgG2a, IgG2b, and IgG3 for mice. The relative abundance of each human subclass is 60% for IgG1, 32% for IgG2, 4% for IgG3, and 4% for IgG4. In an IgG deficiency, there may be a shortage of one or more subclasses (4).

References

1. Painter RH. (1998) Encyclopedia of Immunology (Second Edition). Elsevier. 1208-1211

2. Chapter 9 - Antibodies. (2012) Immunology for Pharmacy. Mosby 70-78

3. Schroeder H, Cavacini, L. (2010) Structure and Function of Immunoglobulins. J Allergy Clin Immunol. 125(2 0 2): S41-S52. PMID: 20176268

4. Vidarsson G, Dekkers G, Rispens T. (2014) IgG subclasses and allotypes: from structure to effector functions. Front Immunol. 5:520. PMID: 25368619

Additional IgG (H+L) Products

Product Documents for Sheep anti-Mouse IgG (H+L) Secondary Antibody [Allophycocyanin]

Product Datasheets

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COA

SDS

Product Specific Notices for Sheep anti-Mouse IgG (H+L) Secondary Antibody [Allophycocyanin]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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