Donkey anti-Goat IgG (H+L) Secondary Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-60656
Conjugate
Catalog #
Key Product Details
Species Reactivity
Goat
Applications
Dot Blot, ELISA, Fluorophore-linked immunosorbent assay, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Donkey IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Donkey anti-Goat IgG (H+L) Secondary Antibody was produced by repeated immunization with goat IgG whole molecule in donkey
Clonality
Polyclonal
Host
Donkey
Isotype
IgG
Description
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey Serum, Goat IgG and Goat Serum.
Store vial at 4C prior to opening. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.
Store vial at 4C prior to opening. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.
Scientific Data Images for Donkey anti-Goat IgG (H+L) Secondary Antibody
Western Blot: Donkey anti-Goat IgG (H+L) Secondary Antibody [NBP2-60656]
Western Blot: Donkey anti-Goat IgG (H+L) Secondary Antibody [NBP2-60656] - Western Blot of Donkey anti-Goat IgG antibody. Lane 1: Goat IgG. Load: 50 ng per lane. Primary antibody: NBP2-60656 incubated overnight at 4C. Secondary antibody: Peroxidase donkey secondary antibody incubated for 30 min at RT; incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 28 and 55 kDa for Goat IgG.Immunocytochemistry/ Immunofluorescence: Donkey anti-Goat IgG (H+L) Secondary Antibody [NBP2-60656]
Immunocytochemistry/Immunofluorescence: Donkey anti-Goat IgG (H+L) Secondary Antibody [NBP2-60656] - Using the DyLight 488 format of this antibody.
Dot Blot: Donkey anti-Goat IgG (H+L) Secondary Antibody [NBP2-60656] - Antigen: Goat IgG. Load: Lane 1 - 200 ng Lane 2 - 66.7 ng Lane 3 - 22.2 ng Lane 4 - 7.41 ng Lane 5 - 2.47 ng. Primary antibody: n/a. Secondary antibody: Donkey anti-Goat IgG Antibody Alkaline Phosphatase Conjugated at 1:1,000 for 60 min at RT. Blocked with blocking buffer for 60 min at RT. Image from the Alkaline Phosphatase version of this antibody.
Applications for Donkey anti-Goat IgG (H+L) Secondary Antibody
Application
Recommended Usage
ELISA
1:20000 - 1:100000
Immunohistochemistry
1:1000 - 1:5000
Western Blot
1:2000 - 1:10000
Application Notes
This product has been tested by ELISA and western blot. This antibody is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lot-to-lot consistency.
Formulation, Preparation, and Storage
Purification
Multi-step
Formulation
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: IgG (H+L)
The 4 IgG subclasses, sharing 95% amino acid identity, include IgG1, IgG2, IgG3, and IgG4 for humans and IgG1, IgG2a, IgG2b, and IgG3 for mice. The relative abundance of each human subclass is 60% for IgG1, 32% for IgG2, 4% for IgG3, and 4% for IgG4. In an IgG deficiency, there may be a shortage of one or more subclasses (4).
References
1. Painter RH. (1998) Encyclopedia of Immunology (Second Edition). Elsevier. 1208-1211
2. Chapter 9 - Antibodies. (2012) Immunology for Pharmacy. Mosby 70-78
3. Schroeder H, Cavacini, L. (2010) Structure and Function of Immunoglobulins. J Allergy Clin Immunol. 125(2 0 2): S41-S52. PMID: 20176268
4. Vidarsson G, Dekkers G, Rispens T. (2014) IgG subclasses and allotypes: from structure to effector functions. Front Immunol. 5:520. PMID: 25368619
Additional IgG (H+L) Products
Product Documents for Donkey anti-Goat IgG (H+L) Secondary Antibody
Product Specific Notices for Donkey anti-Goat IgG (H+L) Secondary Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.
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