Goat anti-Mouse IgG1 Heavy Chain Secondary Antibody [DyLight 405]
Novus Biologicals, part of Bio-Techne | Catalog # NB7508V
Conjugate
Catalog #
Key Product Details
Species Reactivity
Mouse
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
DyLight 405 (Excitation = 400 nm, Emission = 420 nm)
Antibody Source
Polyclonal Goat IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Mouse IgG1
Reactivity Notes
Human reactivity reported from a verified customer review.
Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG1. Cross reactivity to mouse IgA, IgM, IgG2a, IgG2b, IgG2c, IgG3 and IgE is undetectable. Some hybridoma clones may express aberrant immunoglobulin-related peptides that are improperly recognized by this antibody.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Goat anti-Mouse IgG1 Heavy Chain Secondary Antibody [DyLight 405]
Immunocytochemistry/Immunofluorescence: Goat anti-Mouse IgG1 Heavy Chain Secondary Antibody [DyLight 405] [NB7508V] - Human MCF7 cells was stained with primary KDM6A antibody [H00007403-M05] (1:50), followed by Goat anti-Mouse IgG1 Heavy Chain Secondary Antibody [Dylight 405] (NB7508V, 1:1000).
Applications for Goat anti-Mouse IgG1 Heavy Chain Secondary Antibody [DyLight 405]
Application
Recommended Usage
ELISA
1:1000- 1:30000
Immunocytochemistry/ Immunofluorescence
1:200 - 1:2000
Immunohistochemistry
1:200 - 1:2000
Immunohistochemistry-Paraffin
1:200 - 1:2000
Western Blot
1:1000 - 1:30000
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using NB7508V in the following applications:
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
50 mM Sodium Borate
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: IgG1 Heavy Chain
Additional IgG1 Heavy Chain Products
Product Documents for Goat anti-Mouse IgG1 Heavy Chain Secondary Antibody [DyLight 405]
Product Specific Notices for Goat anti-Mouse IgG1 Heavy Chain Secondary Antibody [DyLight 405]
DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.
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