Key Product Details

Species Reactivity

Mouse

Applications

Dot Blot, ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Alkaline Phosphatase

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all IgG2b Heavy Chain Secondary Antibodies »

Product Specifications

Immunogen

Mouse IgG2b heavy chain

Specificity

Specificity was confirmed by ELISA at less than 1% cross reactivity against other mouse heavy or light chain isotypes.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Store vial at 4C before opening. DO NOT FREEZE. This product is stable at 4C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

This product was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine) and anti-Rabbit Serum

Scientific Data Images

Dot Blot: Rabbit anti-Mouse IgG2b Heavy Chain Secondary Antibody [Alkaline Phosphatase] [NBP1-72674] - Load: Lane 1 - 200 ng Lane 2 - 66.7 ng Lane 3 - 22.2 ng Lane 4 - 7.41 ng Lane 5 - 2.47 ng. Incubated for 1 HR at RT; blocked for 1 HR at RT.
Rabbit anti-Mouse IgG2b Heavy Chain Secondary Antibody [Alkaline Phosphatase]

Rabbit anti-Mouse IgG2b Heavy Chain Secondary Antibody [Alkaline Phosphatase]

Dot Blot of Rabbit anti-Mouse IgG2b Antibody Alkaline Phosphatase Conjugated. Antigen: Mouse IgG2b. Load: Lane 1 - 200 ng Lane 2 - 66.7 ng Lane 3 - 22.2 ng Lane 4 - 7.41 ng Lane 5 - 2.47 ng. Primary antibody: n/a. Secondary antibody: Rabbit anti-Rabbit anti-Mouse IgG2b Heavy Chain Secondary Antibody [Alkaline Phosphatase]at 1:1,000 for 1 HR at RT. Block for 1 HR at RT.

Applications

Application
Recommended Usage

ELISA

1:8000 - 1:32000

Immunohistochemistry

1:200 - 1:1000

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

1:1000 - 1:3000
Application Notes
This product has been tested by ELISA and dot blot and is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lot-to-lot consistency.

Formulation, Preparation, and Storage

Purification

Multi-step

Formulation

0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Product Documents

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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