Rabbit anti-Mouse IgM (H+L) Secondary Antibody [FITC]
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-73882

Key Product Details
Species Reactivity
Mouse
Applications
Flow Cytometry, Fluorophore-linked immunosorbent assay, Immunocytochemistry/ Immunofluorescence
Label
FITC (Excitation = 495 nm, Emission = 519 nm)
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Mouse IgM, whole molecule
Specificity
Some light chain cross reactivity with Mouse IgG may occur.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room
This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-Rabbit Serum, Mouse IgM, and Mouse Serum
This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-Rabbit Serum, Mouse IgM, and Mouse Serum
Applications for Rabbit anti-Mouse IgM (H+L) Secondary Antibody [FITC]
Application
Recommended Usage
Flow Cytometry
1:1:10 - 1:1000
Fluorophore-linked immunosorbent assay
1:10000 - 1:50000
Immunocytochemistry/ Immunofluorescence
1:1000 - 1:5000
Application Notes
This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Formulation, Preparation, and Storage
Purification
Multi-step
Formulation
0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: IgM (H+L)
Additional IgM (H+L) Products
Product Documents for Rabbit anti-Mouse IgM (H+L) Secondary Antibody [FITC]
Product Specific Notices for Rabbit anti-Mouse IgM (H+L) Secondary Antibody [FITC]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.
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