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Key Product Details

Species Reactivity

Mouse

Applications

Dot Blot, ELISA, Fluorophore-linked immunosorbent assay, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Mouse IgM whole molecule

Specificity

No reaction was observed against other mouse heavy or light chain proteins.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Description

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgM coupled to agarose followed by solid phase adsorption(s). Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgM and Mouse Serum

Store vial at 4C prior to opening. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.

Scientific Data Images for Goat anti-Mouse IgM Heavy Chain Secondary Antibody

Immunocytochemistry/ Immunofluorescence: Goat anti-Mouse IgM Heavy Chain Secondary Antibody [NB120-9167]

Immunocytochemistry/ Immunofluorescence: Goat anti-Mouse IgM Heavy Chain Secondary Antibody [NB120-9167]

Immunocytochemistry/Immunofluorescence: Goat anti-Mouse IgM Heavy Chain Secondary Antibody [NB120-9167] - Immunocytochemistry/Immunofluorescence: Goat anti-Mouse IgM Secondary Antibody [DyLight 488] [NBP1-72873] Image from the DyLight 488 version of this antibody.
Fluorophore-linked immunosorbent assay: Goat anti-Mouse IgM Heavy Chain Secondary Antibody [NB120-9167]

Fluorophore-linked immunosorbent assay: Goat anti-Mouse IgM Heavy Chain Secondary Antibody [NB120-9167]

Fluorophore-linked immunosorbent assay: Goat anti-Mouse IgM Heavy Chain Secondary Antibody [NB120-9167] - Image using the DyLight 405 format of this antibody.
Fluorophore-linked immunosorbent assay: Goat anti-Mouse IgM Heavy Chain Secondary Antibody [NB120-9167]

Fluorophore-linked immunosorbent assay: Goat anti-Mouse IgM Heavy Chain Secondary Antibody [NB120-9167]

Fluorophore-linked immunosorbent assay: Goat anti-Mouse IgM Heavy Chain Secondary Antibody [NB120-9167] - Image using the DyLight 680 format of this antibody.

Applications for Goat anti-Mouse IgM Heavy Chain Secondary Antibody

Application
Recommended Usage

ELISA

1:20000 - 1:50000

Immunohistochemistry

1:1000 - 1:5000

Western Blot

1:500 - 1:2000
Application Notes
This product has been tested by ELISA, dot blot, and western blot and is suitable for immunoblotting (western or dot blot), ELISA, and immunohistochemistry assays requiring lot-to-lot consistency.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Multi-step

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IgM Heavy Chain

Alternate Names

Constant region of heavy chain of IgM, Ig Mu Chain C Region BOT, Ig Mu Chain C Region GAL, Ig Mu Chain C Region OU, immunoglobulin heavy constant mu, Immunoglobulin mu chain

Gene Symbol

IGHM

Additional IgM Heavy Chain Products

Product Documents for Goat anti-Mouse IgM Heavy Chain Secondary Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Goat anti-Mouse IgM Heavy Chain Secondary Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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