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Key Product Details

Species Reactivity

Human

Applications

Chemiluminescence Immunoassay, ELISA, Flow Cytometry, Immunoassay, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunomicroscopy, Western Blot

Label

PE/Atto594 (Excitation = 488 nm, Emission = 627 nm)

Antibody Source

Polyclonal Goat IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Purified Human IgM, Mu chain

Reactivity Notes

Based on IEP, this antibody reacts with heavy chains on human IgM.

Specificity

Based on Immunoelectrophoresis, this antibody reacts with heavy chains on human IgM. Cross Adsorbed: This antibody was pre-adsorbed to reduce cross-reactivity with proteins of non-related species. Minimal cross-reactivity with human IgA + IgG serum proteins.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Goat anti-Human IgM Heavy Chain Secondary Antibody [PE/Atto594]

Application
Recommended Usage

Chemiluminescence Immunoassay

Optimal dilutions of this antibody should be experimentally determined.

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunoassay

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunomicroscopy

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined. For optimal results using our Tandem dyes, please avoid prolonged exposure to light or extreme temperature fluctuations. These can lead to irreversible degradation or decoupling. When staining intracellular targets, specific attention to the fixation and permeabilization steps in your flow protocol may be required. Please contact our technical support team at technical@novusbio.com if you have any questions.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark. Do not freeze.

Background: IgM Heavy Chain

Alternate Names

Constant region of heavy chain of IgM, Ig Mu Chain C Region BOT, Ig Mu Chain C Region GAL, Ig Mu Chain C Region OU, immunoglobulin heavy constant mu, Immunoglobulin mu chain

Gene Symbol

IGHM

Additional IgM Heavy Chain Products

Product Documents for Goat anti-Human IgM Heavy Chain Secondary Antibody [PE/Atto594]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Goat anti-Human IgM Heavy Chain Secondary Antibody [PE/Atto594]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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