Goat anti-Human Kappa Light Chain Secondary Antibody [DyLight 650]
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-75929
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry, Immunomicroscopy
Label
DyLight 650 (Excitation = 652 nm, Emission = 672 nm)
Antibody Source
Polyclonal Goat IgG
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
Purified Human Kappa light chain
Reactivity Notes
Based on IEP, no reactivity is observed to non-immunoglobulin human serum proteins, heavy chains on all human immunoglobulins, and lambda light chains on all human immunoglobulins
Specificity
Based on IEP, this antibody reacts with kappa and light chains on all human immunoglobulins
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Description
Affinity purified antibody is > 95% based on SDS-PAGE. Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian. DyLight 650 (Ex = 652 nm; Em = 672 nm). After reconstituted, prepare fresh working dilution daily
Product is stable for up to 4 weeks at 2-8C after rehydration. For extended storage after rehydration, add an equal volume of glycerol and store at -20C.
Product is stable for up to 4 weeks at 2-8C after rehydration. For extended storage after rehydration, add an equal volume of glycerol and store at -20C.
Applications for Goat anti-Human Kappa Light Chain Secondary Antibody [DyLight 650]
Application
Recommended Usage
Flow Cytometry
1:20 - 1:2000
Immunomicroscopy
1:20 - 1:2000
Application Notes
This conjugate is suitable for immunomicroscopy and flow cytometry.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Reconstitution
Rehydrate with 0.55 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Formulation
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Preservative
0.05% Sodium Azide
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store lyophilized antibody at 4C. Mix reconstituted liquid with an equal volume of glycerol, aliquot contents and freeze at -20C or below for long term storage.
Background: Kappa Light Chain
Alternate Names
HCAK1, IGKCD, immunoglobulin kappa constant, Km, MGC111575, MGC62011, MGC72072, MGC88770, MGC88771, MGC88809
Gene Symbol
IGKC
Additional Kappa Light Chain Products
Product Documents for Goat anti-Human Kappa Light Chain Secondary Antibody [DyLight 650]
Product Specific Notices for Goat anti-Human Kappa Light Chain Secondary Antibody [DyLight 650]
DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.
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