Detects heavy ( gamma) chains on sheep IgG and light chains on all sheep immunoglobulins, based on IEP. No cross-reactivity is observed with non-immunoglobulin sheep serum proteins or IgG from human, mouse or rabbit, based on IEP. VisUCyte™ HRP Polymer provides high sensitivity with low background in IHC.

Clonality

Polyclonal

Host

Donkey

Isotype

IgG

Scientific Data Images for Sheep IgG VisUCyte HRP Polymer Antibody

Detection of Progesterone R/NR3C3 in Human Breast Cancer Tissue.

Progesterone R/NR3C3 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Sheep Anti-Human Progesterone R/NR3C3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5415) with Anti-Sheep IgG VisUCyte HRP Polymer Antibody (Catalog # VC006). Tissue was stained with DAB (brown color) and counterstained with hematoxylin (blue color). View our protocol for theVisUCyte HRP Polymer Detection Reagent.

Applications for Sheep IgG VisUCyte HRP Polymer Antibody

Application

Recommended Usage

Immunohistochemistry

Prediluted, ready to use with formalin fixed paraffin-embedded and frozen sections. Compatible with most commonly used fixatives.

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Supplied as a solution in PBS with preservative.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

6 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG

VisUCyte™ HRP Polymer is a biotin-free detection reagent which overcomes problems of avidin-biotin detection chemistry, such as endogenous biotin staining and the need to do additional quenching of endogenous biotin and avidin present in some tissues. With VisUCyte HRP Polymer it is possible to achieve specific staining in cells and tissue much faster than the conventional avidin-biotin-HRP procedure. Due the high sensitivity of VisUCyte™ HRP Polymer, the working concentration of primary antibodies may be 2-3 times lower compared to the conventional avidin-biotin-HRP protocol.

Long Name

Immunoglobulin G

Alternate Names

Immunoglobulin G, ImmunoglobulinG

Additional IgG Products

Product Documents for Sheep IgG VisUCyte HRP Polymer Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Sheep IgG VisUCyte HRP Polymer Antibody

For research use only

Citations
Reviews

Sheep IgG VisUCyte HRP Polymer Antibody

R&D Systems, part of Bio-Techne | Catalog # VC006

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all IgG Secondary Antibodies »

Product Specifications

Immunogen

Specificity