Non-Human Primate TIMP-2 Cartridge

Name: Non-Human Primate TIMP-2 Cartridge
Brand: ProteinSimple
SKU: SPCKB-NP-011336

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-NP-011336

Simple Plex Non-Human Primate TIMP-2 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)
Sensitivity:	4.8 pg/mL
Assay Range:	20.48 - 12,500 pg/mL (Serum, EDTA Plasma, Heparin Plasma)
Non-Human Primate TIMP-2 Cartridge Summary:	Simple Plex assay for the detection of non-human primate Tissue Inhibitor of Metalloproteinases 2 (TIMP-2) in serum and plasma (EDTA/Heparin).

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Non-Human Primate TIMP-2 Cartridge

Technical Data
Citations

Product Specifications for Non-Human Primate TIMP-2 Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Non-human Primate
Specificity	Natural and recombinant TIMP-2.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	47	47
Mean (pg/mL)	183	9932	167	10585
Standard Deviation	8.3	587	11.2	991
CV%	4.5	5.9	6.7	9.4

Linearity

Samples containing and/or spiked with high concentrations of TIMP-2 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data Examples for Non-Human Primate TIMP-2 Cartridge

	Non-Human Primate TIMP-2 Ella Assay Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: TIMP-2

TIMPs-1 through -4 regulate the activity of zinc metalloproteases known as MMPs, ADAMs and ADAMTSs. Structurally, TIMPs contain two domains. The N-terminal domain binds to the active site of mature metalloproteases via a 1:1 non-covalent interaction, blocking access of substrates to the catalytic site. In addition, The C-terminal domain of TIMP-1 and TIMP-2 binds to the hemopexin- like domain of pro-MMP-9 and pro-MMP-2, respectively. The latter binding is essential for the cell surface activation of MMP-2 by MMP-14.

Item	Value
Long Name	Tissue Inhibitors of Metalloproteinases 2
Entrez Gene IDs	7077 (Human); 21858 (Mouse); 29543 (Rat)
Alternate Names	CSC-21Ktissue inhibitor of metalloproteinase 2, TIMP metallopeptidase inhibitor 2, TIMP-2, TIMP2, Tissue inhibitor of metalloproteinases 2, metalloproteinase inhibitor 2

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Non-Human Primate TIMP-2 Cartridge

Product Datasheets

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Protocols & Manuals

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