Simple Plex Human Angiopoietin-like 3 Cartridge

Name: Simple Plex Human Angiopoietin-like 3 Cartridge
Brand: ProteinSimple
SKU: SPCKB-PS-008169

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-008169

Simple Plex Human Angiopoietin-like Protein 3/ANGPTL3 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Cell Culture Supernates (10 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)
Sensitivity:	4.21 pg/mL
Assay Range:	15.7 - 24,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Simple Plex Human Angiopoietin-like 3 Cartridge Summary:	Simple Plex assay for the detection of human Angiopoietin-like 3 (ANGPTL3) in cell culture supernatant (CCS), serum and plasma (EDTA/Heparin).

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Human Angiopoietin-like 3 Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Human Angiopoietin-like 3 Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Specificity	Natural and recombinant human ANGPTL3.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Supernates (n=4)	105	101-109

Linearity

Samples containing and/or spiked with high concentrations of human ANGPTL3 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data Examples for Simple Plex Human Angiopoietin-like 3 Cartridge

	Human Angiopoietin-like 3 Ella Assay Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Angiopoietin-like Protein 3/ANGPTL3

Angiopoietin-like, also known as angiopoietin-related proteins, are structurally related to the angiopoietins. They are secreted proteins with N-terminal coiled-coil domains and C-terminal fibrinogen-like domains. Unlike the angiopoietins, ANGPTLs do not bind Tie-2. Seven ANGPTL proteins have been identified (ANGPTL1 through 7).

Item	Value
Entrez Gene IDs	27329 (Human); 30924 (Mouse); 102136264 (Cynomolgus Monkey)
Alternate Names	AGNPT5, ANGPT5angiopoietin-5, ANGPTL3, Ang-5, Angiopoietin-5, Angiopoietin-like Protein 3, FHBL2, angiopoietin 5, angiopoietin-like 3, angiopoietin-related protein 3

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Simple Plex Human Angiopoietin-like 3 Cartridge

Product Datasheets

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Protocols & Manuals

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