Simple Plex Human CCL26/Eotaxin-3 Cartridge

Name: Simple Plex Human CCL26/Eotaxin-3 Cartridge
Brand: ProteinSimple
SKU: SPCKB-PS-000486

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000486

Simple Plex Human CCL26/Eotaxin-3 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD28, and wash buffer.

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Key Product Details

Sample Type & Volume Required Per Well:	Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)
Sensitivity:	0.17 pg/mL
Assay Range:	0.36 - 3,400 pg/mL (Serum, EDTA Plasma, Heparin Plasma)
Simple Plex Human CCL26/Eotaxin-3 Cartridge Summary:	Simple Plex assay for the detection of human Eotaxin-3 in serum and plasma (EDTA/Heparin).

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Human CCL26/Eotaxin-3 Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Human CCL26/Eotaxin-3 Cartridge

Reactivity	Human
Specificity	Natural and recombinant human Eotaxin-3.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	38	38
Mean (pg/mL)	20.2	959	21.2	952
Standard Deviation	1.17	85.3	2.06	101
CV%	5.8	8.9	9.7	10.6

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
EDTA Plasma (n=4)	108	103-113
Heparin Plasma (n=4)	108	99-112
Serum (n=4)	105	85-119

Linearity

Samples containing and/or spiked with high concentrations of human Eotaxin-3 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data Examples for Simple Plex Human CCL26/Eotaxin-3 Cartridge

	Human Eotaxin-3 Simple Plex Assay Standard Curve

Preparation and Storage

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CCL26/Eotaxin-3

Item	Value
Entrez Gene IDs	10344 (Human)
Alternate Names	C-C motif chemokine 26, CC chemokine IMAC, CCL26, Eotaxin-3, Eotaxin3, IMAC, MGC126714, MIP-4a, MIP-4alpha, Macrophage inflammatory protein 4-alpha, SCYA26, SCYA26MIP-4-alpha, Small-inducible cytokine A26, TSC-1Thymic stroma chemokine-1, chemokine (C-C motif) ligand 26, chemokine N1, small inducible cytokine A26, small inducible cytokine subfamily A (Cys-Cys), member 26

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Simple Plex Human CCL26/Eotaxin-3 Cartridge

Product Datasheets

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COA

Protocols & Manuals

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