Simple Plex Human gp130 Cartridge

Name: Simple Plex Human gp130 Cartridge
Brand: ProteinSimple
SKU: SPCKB-PS-000494

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000494

Product Datasheet / COA

Simple Plex Human GP130 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Cell Culture Supernates (25 uL), Serum (500 nL), EDTA Plasma (500 nL), Heparin Plasma (500 nL), Urine (5 uL)
Sensitivity:	0.92 pg/mL
Assay Range:	6.19 - 23,640 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)
Simple Plex Human gp130 Cartridge Summary:	Simple Plex assay for the detection of human gp130 in cell culture supernatant (CCS), serum, plasma (EDTA/Heparin), and urine.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Human gp130 Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Human gp130 Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Human
Specificity	Natural and recombinant human gp130.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Supernates (n=2)	107	107-108

Linearity

Samples containing and/or spiked with high concentrations of human gp130 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data Examples for Simple Plex Human gp130 Cartridge

	Human gp130 Ella Assay Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: gp130

Gp130, the common signal transducing receptor component shared by the functional receptor complexes of the IL-6 family of cytokines, belongs to the classI cytokine receptor family. Binding of IL-6 or IL-11 to either the membrane-anchored or soluble IL-6 R or IL-11 R, respectively, initiates the association of IL-6 R (IL-11 R) with gp130. The complex then undergoes homodimerization and signal transduction. For other IL-6 family cytokines, such as LIF and OSM, signal transduction is similarly triggered by the heterodimerization of gp130 with LIFR or OSM R.

Item	Value
Long Name	Glycoprotein 130
Entrez Gene IDs	3572 (Human); 16195 (Mouse); 25205 (Rat); 101926138 (Cynomolgus Monkey)
Alternate Names	CD130, CD130 antigen, CDw130, DKFZp564F053, IL-6 receptor subunit beta, IL-6R subunit beta, IL-6R-beta, IL-6RB, IL6ST, Interleukin-6 signal transducer, Membrane glycoprotein 130, Oncostatin-M receptor subunit alpha, gp130, gp130 of the rheumatoid arthritis antigenic peptide-bearing soluble form, interleukin 6 signal transducer (gp130, oncostatin M receptor), interleukin receptor beta chain, interleukin-6 receptor subunit beta, membrane glycoprotein gp130

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Simple Plex Human gp130 Cartridge

Product Datasheets

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COA

Protocols & Manuals

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