Simple Plex Human ST2/IL-33R/IL-1R4 Cartridge

Name: Simple Plex Human ST2/IL-33R/IL-1R4 Cartridge
Brand: ProteinSimple
SKU: SPCKB-PS-000221

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000221

Simple Plex Human ST2/IL-33R/IL-1R4 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.

Need a different panel or sample type? Customize your cartridge here.

Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Cell Culture Supernates (10 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)
Sensitivity:	8.72 pg/mL
Assay Range:	36.9 - 56,350 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Simple Plex Human ST2/IL-33R/IL-1R4 Cartridge Summary:	Simple Plex assay for the detection of human ST2 in cell culture supernatant, serum, and plasma (EDTA/Heparin)

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Human ST2/IL-33R/IL-1R4 Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Human ST2/IL-33R/IL-1R4 Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Human
Specificity	Natural and recombinant human ST2.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	24	22
Mean (pg/mL)	141	7745	151	7857
Standard Deviation	2.4	231	17.7	287
CV%	1.7	3	11.7	3.6

Recovery

Recovery was not necessary as ST2 shows natural linearity data from a neat sample to a 1:16 dilution.

Sample Type	Average % Recovery	Range %
Cell Culture Samples (n=3)	103	101-106

Linearity

Samples containing and/or spiked with high concentrations of human ST2 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data Examples for Simple Plex Human ST2/IL-33R/IL-1R4 Cartridge

	Human ST2 Ella Assay Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: ST2/IL-33R

ST2, also known as IL-1 R4, is a component of the receptor for the proinflammatory cytokine Interleukin-33. The IL-33 receptor additionally includes IL-1 RAcp which also associates with IL-1 RI and IL-1 R rp2/IL-1 R6. A soluble form of ST2 functions as a decoy receptor. IL-33 promotes Th2-biased immune responses, resulting in eosinophilia, mast cell activation, and allergic inflammation. In addition, IL-33 enhances inflammation-associated hypernociception and protects from atherosclerosis and cardiac myocyte hypertrophy. Soluble ST2 is elevated in the serum under inflammatory conditions including allergic asthma, sepsis, trauma, dengue fever, pulmonary disease, and lupus. It is also associated aortic stenosis, congestive cardiomyopathy, and risk of cardiovascular heart failure and death.

Item	Value
Long Name	Interleukin 33 Receptor
Entrez Gene IDs	9173 (Human); 17082 (Mouse)
Alternate Names	DER4, DER4ST2growth stimulation-expressed, Fit-1, IL-1 R4, IL-1R4, IL-1RL1, IL-33 R, IL-33R, IL1R4, IL33R, Ly84, MGC32623, Protein ST2, ST2, ST2L, ST2V, T1, T1homolog of mouse growth stimulation-expressed, interleukin 1 receptor-like 1, interleukin 1 receptor-related protein, interleukin-1 receptor-like 1

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Simple Plex Human ST2/IL-33R/IL-1R4 Cartridge

Product Datasheets

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Protocols & Manuals

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