Simple Plex Mouse Angiopoietin-like 3 Cartridge

Name: Simple Plex Mouse Angiopoietin-like 3 Cartridge
Brand: ProteinSimple
SKU: SPCKB-MP-007716

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-MP-007716

Simple Plex Mouse Angiopoietin-like 3 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD09, and wash buffer.

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Cell Culture Supernates (5 uL), Serum (500 nL), EDTA Plasma (500 nL), Heparin Plasma (500 nL)
Sensitivity:	5.66 pg/mL
Assay Range:	15.7 - 240,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Simple Plex Mouse Angiopoietin-like 3 Cartridge Summary:	Simple Plex assay for the detection of mouse ANGPTL3 in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin).

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Mouse Angiopoietin-like 3 Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Mouse Angiopoietin-like 3 Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Mouse
Specificity	Natural and recombinant mouse Angiopoietin-like 3.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	60	60
Mean (pg/mL)	146	7688	136	7884
Standard Deviation	6.51	505	16	932
CV%	4.5	6.6	11.7	11.8

Linearity

Samples containing and/or spiked with high concentrations of mouse ANGPTL3 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data Examples for Simple Plex Mouse Angiopoietin-like 3 Cartridge

	Mouse Angiopoietin-like 3 Simple Plex Assay Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Angiopoietin-like Protein 3/ANGPTL3

Angiopoietin-like, also known as angiopoietin-related proteins, are structurally related to the angiopoietins. They are secreted proteins with N-terminal coiled-coil domains and C-terminal fibrinogen-like domains. Unlike the angiopoietins, ANGPTLs do not bind Tie-2. Seven ANGPTL proteins have been identified (ANGPTL1 through 7).

Item	Value
Entrez Gene IDs	27329 (Human); 30924 (Mouse); 102136264 (Cynomolgus Monkey)
Alternate Names	AGNPT5, ANGPT5angiopoietin-5, ANGPTL3, Ang-5, Angiopoietin-5, Angiopoietin-like Protein 3, FHBL2, angiopoietin 5, angiopoietin-like 3, angiopoietin-related protein 3

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Simple Plex Mouse Angiopoietin-like 3 Cartridge

Product Datasheets

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Protocols & Manuals

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