Simple Plex Mouse CCL3/MIP-alpha Cartridge

Name: Simple Plex Mouse CCL3/MIP-alpha Cartridge
Brand: ProteinSimple
SKU: SPCKB-MP-007987

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-MP-007987

Simple Plex Mouse CCL3/MIP-1 alpha assay kit for use on Ella instrument. Contains cartridge, sample diluent SD09, and wash buffer.

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Key Product Details

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Mouse CCL3/MIP-alpha Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Mouse CCL3/MIP-alpha Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Mouse
Specificity	Natural and recombinant mouse MIP-1 alpha.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Linearity

Scientific Data Examples for Simple Plex Mouse CCL3/MIP-alpha Cartridge

	Mouse CCL3/MIP-1 alpha Ella Assay Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CCL3/MIP-1 alpha

MIP-1 alpha (macrophage inflammatory protein 1 alpha) is a member of the CC or beta chemokine subfamily that was originally purified from the conditioned media of an LPS-stimulated murine macrophage cell line. MIP-1 alpha acts as a chemoattractant to a variety of cells including monocytes, T cells, B cells and eosinophils.

The two human MIP-1 alpha genes arise by duplication/mutation. They code for MIP-1 alpha isoforms CCL3/LD78a and CCL3L1/LD78b, which share 94% amino acid sequence homology. Whereas the human CCL3/LD78a is a single-copy gene, the human CCL3L1/LD78b gene copy number varies within the population. Human CCL3L1/LD78b binds and signals through chemokine receptors CCR1, CCR5. When compared to CCL3/LD78a, CCL3L1/LD78b has higher binding affinity to CCR5, which also functions as a coreceptor for HIV-1 entry. The copy number of CCL3L1 is one of several genetic determinants of HIV-1 susceptibility.

Item	Value
Entrez Gene IDs	6348 (Human); 20302 (Mouse); 25542 (Rat); 448787 (Canine); 102136134 (Cynomolgus Monkey)
Alternate Names	C-C motif chemokine 3, MIP1-(a), AI323804, CCL3, G0S19-1, LD78a, LD78alpha, MIP-1 alpha, MIP-1alpha, MIP1 alpha, MIP1-alpha, MIP1A, PAT 464.1, SCYA3, SIS-beta, chemokine (C-C motif) ligand 3

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Simple Plex Mouse CCL3/MIP-alpha Cartridge

Product Datasheets

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Protocols & Manuals

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