Simple Plex Mouse/Rat Chitinase 3-like 1/YKL-40 Cartridge

Name: Simple Plex Mouse/Rat Chitinase 3-like 1/YKL-40 Cartridge
Brand: ProteinSimple
SKU: SPCKB-MP-008843

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-MP-008843

Simple Plex Mouse/Rat Chitinase 3-like 1 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD10, and wash buffer.

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Mouse Bronchoalveolar Lavage (10 uL), Mouse Serum (10 uL), Rat Serum (10 uL), Mouse EDTA Plasma (10 uL), Rat EDTA Plasma (10 uL), Mouse Heparin Plasma (10 uL), Rat Heparin Plasma (10 uL), Mouse Cerebrospinal Fluid (10 uL)
Sensitivity:	1.08 pg/mL
Assay Range:	4.06 - 15,502 pg/mL (Mouse Bronchoalveolar Lavage, Cell Culture Supernates, Mouse Serum, Rat Serum, Mouse EDTA Plasma, Mouse Heparin Plasma, Mouse Cerebrospinal Fluid)
Simple Plex Mouse/Rat Chitinase 3-like 1/YKL-40 Cartridge Summary:	Simple Plex assay for the detection of mouse and rat Chitinase 3-like 1 (CHI3L1) in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin), bronchoalveolar lavage fluid (BALF), and cerebrospinal fluid (CSF).

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Mouse/Rat Chitinase 3-like 1/YKL-40 Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Mouse/Rat Chitinase 3-like 1/YKL-40 Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Mouse, Rat
Specificity	Natural and recombinant mouse and rat CHI3L1.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Mouse Bronchoalveolar Lavage, Cell Culture Supernates, Mouse Serum, Rat Serum, Mouse EDTA Plasma, Mouse Heparin Plasma, Mouse Cerebrospinal Fluid

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	41	43
Mean (pg/mL)	116	559	101	5135
Standard Deviation	3.64	439	10.8	469
CV%	3.1	7.8	10.7	9.1

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=2)	116	115-117

Linearity

Scientific Data Examples for Simple Plex Mouse/Rat Chitinase 3-like 1/YKL-40 Cartridge

	Mouse/Rat CH3L1 Ella Assay Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Chitinase 3-like 1/YKL-40

Chitinase 3-like 1 (CHI3L1), also called BRP39 in mouse or YKL-40 in humans, is a 39 kDa glycoprotein member of the glycosyl hydrolase 18 family although it does not show chitotriosidase activity. CHI3L1 is expressed by articular chondrocytes, synovial cells, activated monocyte-derived macrophages, neutrophils, endothelial cells, vascular smooth muscle cells, and some cancer cells. CHI3L1 binds to chitin and heparins, and it enhances cell adhesion, proliferation and tumor angiogenesis. Circulating CHI3L1 is elevated during inflammation and connective tissue remodeling such as arthritis, chronic obstructive pulmonary disease, diabetes, cardiovascular disease, inflammatory bowel disease, and liver cirrhosis. It is frequently upregulated in glioblastoma, myxoid chondrosarcoma, melanoma and carcinomas of the breast, thyroid, colon, lung, kidney, and ovary.

Item	Value
Entrez Gene IDs	1116 (Human); 12654 (Mouse); 102138036 (Cynomolgus Monkey)
Alternate Names	AW208766, Brp39, CHI3L1, Chitinase 3 like 1, Chitinase 3-like 1, HCgp39, YKL-40, gp39

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Simple Plex Mouse/Rat Chitinase 3-like 1/YKL-40 Cartridge

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