3-Mercaptopicolinic acid hydrochloride

Name: 3-Mercaptopicolinic acid hydrochloride
Brand: Tocris Bioscience
SKU: 7577

Tocris Bioscience, part of Bio-Techne | Catalog # 7577

COA / SDS

Phospoenolpyruvate carboxykinase (PEPCK) inhibitor; inhibits gluconeogenesis

Key Product Details

Description:	Phospoenolpyruvate carboxykinase (PEPCK) inhibitor; inhibits gluconeogenesis
Chemical Name:	3-Mercapto-2-pyridinecarboxylic acid hydrochloride
Molecular Weight:	191.63

View Full Technical Details for 3-Mercaptopicolinic acid hydrochloride

Technical Data
Citations

Biological Activity for 3-Mercaptopicolinic acid hydrochloride

3-Mercaptopicolinic acid hydrochloride is a phospoenolpyruvate carboxykinase (PEPCK) inhibitor (IC₅₀ = 7.5 μM at hPEPCK). Inhibits glucose synthesis. In vitro, 3-Mercaptopicolinic acid reduces proliferation of T_reg cells, induces myogenic differentiation of C2C12 cells, and reduces colony growth formation of MCF7 cells. Also sensitizes melanoma cells to Vemurafenib (Cat. No. 7309) via inhibition of cytoplasmic PEPCK.

Technical Data for 3-Mercaptopicolinic acid hydrochloride

M. Wt	191.63
Formula	C₆H₅NO₂S.HCl
Storage	Store at -20°C
CAS Number	320386-54-7
PubChem ID	24721311
InChi Key	DYGYEUUVNLEHJP-UHFFFAOYSA-N
Smiles	O=C(C1=NC=CC=C1S)O.Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for 3-Mercaptopicolinic acid hydrochloride

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	DMSO	3.83	20
	water	1.92	10

Preparing Stock Solutions for 3-Mercaptopicolinic acid hydrochloride

The following data is based on the product molecular weight 191.63.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.2 mM	26.09 mL	130.46 mL	260.92 mL
1 mM	5.22 mL	26.09 mL	52.18 mL
2 mM	2.61 mL	13.05 mL	26.09 mL
10 mM	0.52 mL	2.61 mL	5.22 mL

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References for 3-Mercaptopicolinic acid hydrochloride

References are publications that support the biological activity of the product.

Ren Overcoming chemoresistance to b-raf inhibitor in melanoma via targeted inhibition of phosphoenolpyruvate carboxykinase1 using 3-mercaptopropionic acid. Bioengineered
Aragó Pharmacology and preclinical validation of a novel anticancer compound targeting PEPCK-M. Biomed.Pharmacother. 2020 PMID: 31739159
Mcleod Characterization of 3-[(carboxymethyl)thio]picolinic acid: a novel inhibitor of phosphoenolpyruvate carboxykinase. Biochemistry 2019 PMID: 31461616
Brearley The phosphoenolpyruvate carboxykinase (PEPCK) inhibitor, 3-mercaptopicolinic acid (3-MPA), induces myogenic differentiation in C2C12 cells. Sci.Rep. 2020 PMID: 33335245
Watson Metabolic support of tumour-infiltrating regulatory T cells by lactic acid. Nature 2021 PMID: 33589820