3-Methyladenine

Name: 3-Methyladenine
Brand: Tocris Bioscience
SKU: 3977

Tocris Bioscience, part of Bio-Techne | Catalog # 3977

Citations (25)

COA / SDS

Class III PI 3-kinase inhibitor; also inhibits autophagy

Key Product Details

Description:	Class III PI 3-kinase inhibitor; also inhibits autophagy
Alternative Names:	3-MA
Chemical Name:	3-Methyl-3H-purin-6-amine
Purity:	≥99% (HPLC)
Molecular Weight:	149.15

View Full Technical Details for 3-Methyladenine

Technical Data
Citations

Biological Activity for 3-Methyladenine

3-Methyladenine is an inhibitor of class III phosphatidylinositol 3-kinase (PI 3-kinase); also inhibits the autophagic sequestration of cell proteins in rat hepatocytes. Blocks apoptosis in cerebellar granule cells (CGCs) following serum and potassium deprivation.

Compound Libraries for 3-Methyladenine

3-Methyladenine is also offered as part of the Tocriscreen Kinase Inhibitor Library, Tocriscreen Antiviral Library and Tocriscreen Epigenetics Library. Find out more about compound libraries available from Tocris.

Technical Data for 3-Methyladenine

M. Wt	149.15
Formula	C₆H₇N₅
Storage	Store at +4°C
Purity	≥99% (HPLC)
CAS Number	5142-23-4
PubChem ID	1673
InChi Key	FSASIHFSFGAIJM-UHFFFAOYSA-N
Smiles	NC(N=CN2C)=C1C2=NC=N1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for 3-Methyladenine

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	2.98	20

Preparing Stock Solutions for 3-Methyladenine

The following data is based on the product molecular weight 149.15.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.2 mM	33.52 mL	167.62 mL	335.23 mL
1 mM	6.70 mL	33.52 mL	67.05 mL
2 mM	3.35 mL	16.76 mL	33.52 mL
10 mM	0.67 mL	3.35 mL	6.70 mL

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Reconstitution Calculator

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References for 3-Methyladenine

References are publications that support the biological activity of the product.

Canu Role of the autophagic-lysosomal system on low potassium-induced apoptosis in cultured cerebellar granule cells. J.Neurochem. 2005 PMID: 15715672
Seglen and Gordon 3-methyladenine: specific inhibitor of autophagic/lysosomal protein degradation in isolated rat hepatocytes. Proc.Natl.Acad.Sci.U.S.A. 1982 PMID: 6952238
Blommaart The phosphatidylinositol 3-kinase inhibitors wortmannin and LY294002 inhibit autophagy in isolated rat hepatocytes. Eur.J.Biochem. 1997 PMID: 9030745