7-Aminoactinomycin D Solution

Flow Cytometry

7-Aminoactinomycin D Solution
Molecular Weight 1270.43

Fluorescent DNA stain
7- Amino-Actinomycin D (7-AAD) can be used as a viability probe for methods of nonviable cell exclusion based on light scatter and uptake of the reagent as detected in FL3. 7-AAD is excluded by viable cells but can penetrate cell membranes of dying or dead cells, in which it intercalates into double stranded nucleic acids. It can be used in place of Propidium Iodide (PI) for omission of nonviable cells in flow cytometric assay. The advantage of 7-AAD over PI is that detection is in the far red range of the spectrum (650 nm long-pass filter) and is read in the FL3, compared to PI detection in FL2. This allows this solution to be used in combination with PE (phycoerythrin), FITC (fluorescein isothiocyanate), and APC (allophycocyanin) conjugated antibodies, as well as any other conjugated antibody that does not emit into FL3.

PBS and 1.0% FBS

0.09% Sodium Azide

Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Store at 4C in the dark.