A01

Name: A01
Brand: Tocris Bioscience
SKU: 5397

Tocris Bioscience, part of Bio-Techne | Catalog # 5397

Citations (25)

COA / SDS

High affinity Smurf1 inhibitor; enhances BMP signaling

Key Product Details

Description:	High affinity Smurf1 inhibitor; enhances BMP signaling
Chemical Name:	[4-[[4-Chloro-3-(trifluoromethyl)phenyl]sulfonyl]-1-piperazinyl][4-(5-methyl-1H-pyrazol-1-yl)phenyl]methanone
Purity:	≥98% (HPLC)
Molecular Weight:	512.93

View Full Technical Details for A01

Technical Data
Citations

Biological Activity for A01

A01 is a high affinity Smurf1 E3 ubiquitin ligase inhibitor (K_d = 3.7 nM). Attenuates Smurf1-mediated Smad 1/5 degradation and enhances BMP signaling. Potentiates BMP-2-induced osteoblastic activity in C2C12 myoblasts and MC3T3-E1 osteoblast precursor cells.

Technical Data for A01

M. Wt	512.93
Formula	C₂₂H₂₀ClF₃N₄O₃S
Storage	Store at +4°C
Purity	≥98% (HPLC)
CAS Number	1007647-73-5
PubChem ID	17555704
InChi Key	QFYLTUDRXBNZFQ-UHFFFAOYSA-N
Smiles	ClC1=C(C(F)(F)F)C=C(S(N2CCN(C(C3=CC=C(N4C(C)=CC=N4)C=C3)=O)CC2)(=O)=O)C=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for A01

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	51.29	100

Preparing Stock Solutions for A01

The following data is based on the product molecular weight 512.93.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.95 mL	9.75 mL	19.50 mL
5 mM	0.39 mL	1.95 mL	3.90 mL
10 mM	0.19 mL	0.97 mL	1.95 mL
50 mM	0.04 mL	0.19 mL	0.39 mL

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References for A01

References are publications that support the biological activity of the product.

Cao Selective small molecule compounds increase BMP-2 responsiveness by inhibiting Smurf1-mediated Smad1/5 degradation. Sci.Rep. 2014 PMID: 24828823

A01