A12B4C3

Name: A12B4C3
Brand: Tocris Bioscience
SKU: 5097

Tocris Bioscience, part of Bio-Techne | Catalog # 5097

Citations (1)

COA / SDS

Potent and selective polynucleotide kinase/phosphatase (PNKP) inhibitor

Key Product Details

Description:	Potent and selective polynucleotide kinase/phosphatase (PNKP) inhibitor
Chemical Name:	4a,7a-Dihydro-2-(1-hydroxyundecyl)-1-[(4-nitrophenyl)amino]-6-phenyl-1H-pyrrolo[3,4-b]pyridine-5,7(2H,6H)-dione
Purity:	≥98% (HPLC)
Molecular Weight:	534.65

View Full Technical Details for A12B4C3

Technical Data
Citations

Biological Activity for A12B4C3

A12B4C3 is a potent and selective polynucleotide kinase/phosphatase (PNKP) inhibitor (IC₅₀ = 60 nM). Displays no effect on calcineurin, protein PP-1 or APTX. Interrupts DNA repair and enhances radiosensitivity of human A549 lung carcinoma and MDA-MB-231 cells.

Compound Libraries for A12B4C3

A12B4C3 is also offered as part of the Tocriscreen Kinase Inhibitor Library. Find out more about compound libraries available from Tocris.

Technical Data for A12B4C3

M. Wt	534.65
Formula	C₃₀H₃₈N₄O₅
Storage	Store at +4°C
Purity	≥98% (HPLC)
CAS Number	1005129-80-5
PubChem ID	6610064
InChi Key	KVHGJAKTBPFFNV-UHFFFAOYSA-N
Smiles	O=C2N(C4=CC=CC=C4)C(C1C=CC(C(O)CCCCCCCCCC)N(NC3=CC=C([N+]([O-])=O)C=C3)C12)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for A12B4C3

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	53.47	100

Preparing Stock Solutions for A12B4C3

The following data is based on the product molecular weight 534.65.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.87 mL	9.35 mL	18.70 mL
5 mM	0.37 mL	1.87 mL	3.74 mL
10 mM	0.19 mL	0.94 mL	1.87 mL
50 mM	0.04 mL	0.19 mL	0.37 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.

Mass

Concentration

Volume

M. Wt*

g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Dilution Calculator

Calculate the dilution required to prepare a stock solution.

Concentration 1 (C1)

Volume 1 (V1)

Concentration 2 (C2)

Volume 2 (V2)

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

References for A12B4C3

References are publications that support the biological activity of the product.

Freschauf Mechanism of action of an imidopiperidine inhibitor of human polynucleotide kinase/phosphatase. J.Biol.Chem. 2010 PMID: 19940137
Schellenberg DNA end processing by polynucleotide kinase/phosphatase. Proc.Natl.Acad.Sci.U.S.A. 2011 PMID: 22184240
Freschauf Identification of a small molecule inhibitor of the human DNA repair enzyme polynucleotide kinase/phosphatase. Cancer Res. 2009 PMID: 19773431