Chloroquine diphosphate

Name: Chloroquine diphosphate
Brand: Tocris Bioscience
SKU: 4109

Tocris Bioscience, part of Bio-Techne | Catalog # 4109

Citations (22)

COA / SDS

Antimalarial; inhibits apoptosis and autophagy

Key Product Details

Description:	Antimalarial; inhibits apoptosis and autophagy
Chemical Name:	N⁴-(7-Chloro-4-quinolinyl)-N¹,N¹-dimethyl-1,4-pentanediamine diphosphate salt
Purity:	≥99% (HPLC)
Molecular Weight:	515.86

View Full Technical Details for Chloroquine diphosphate

Technical Data
Citations

Biological Activity for Chloroquine diphosphate

Chloroquine diphosphate is an antimalarial (Plasmodium) medicine. Inhibits cell growth and induces cell death in numerous cancer cell lines; inhibits cell proliferation and viability and induces apoptosis in 4T1 mouse breast cancer cells in vitro. Exhibits antimetastatic activity. Also inhibits autophagy via a mechanism distinct from that of 3-methyladenine (Cat. No. 3977). Blocks receptor-mediated endocytosis of mannose-glycoconjugates by macrophages. Inhibits SARS-CoV-2 infection in vitro (EC₅₀ = 1.13 μM). Chloroquine improves efficacy of adeno-associated viral gene transduction in vivo and in vitro, as well as enhancing non-viral gene transfection in breast cancer cells.

Tocris products are for biomedical research use only. They are not intended for human or veterinary use.

Technical Data for Chloroquine diphosphate

M. Wt	515.86
Formula	C₁₈H₂₆ClN₃·2H₃PO₄
Storage	Desiccate at RT
Purity	≥99% (HPLC)
CAS Number	50-63-5
PubChem ID	64927
InChi Key	QKICWELGRMTQCR-UHFFFAOYSA-N
Smiles	CCN(CC)CCCC(C)NC2=CC=NC1=CC(Cl)=CC=C12.O=P(O)(O)O.O=P(O)(O)O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Chloroquine diphosphate

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	water	51.59	100

Preparing Stock Solutions for Chloroquine diphosphate

The following data is based on the product molecular weight 515.86.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.94 mL	9.69 mL	19.39 mL
5 mM	0.39 mL	1.94 mL	3.88 mL
10 mM	0.19 mL	0.97 mL	1.94 mL
50 mM	0.04 mL	0.19 mL	0.39 mL

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References for Chloroquine diphosphate

References are publications that support the biological activity of the product.

McErlean Rational design and characterisation of a linear cell penetrating peptide for non-viral gene delivery. J.Control.Release 2021 PMID: 33227336
Chandler Enhancement of adeno-associated cirus-mediated gene therapy using hydroxychloroquine in murine and human tissues. Mol.Ther.Methods Clin.Dev. 2019 PMID: 31309129
Tietze Chloroquine and ammonium ion inhibit receptor-mediated endocytosis of mannose-glycoconjugates by macrophages: Apparent inhibition of receptor recycling. Biochem.Biophys.Res.Commun. 1980 PMID: 7378072
Wang Remdesivir and chloroquine effectively inhibit the recently emerged novel coronavirus (2019-nCoV) in vitro. Cell Research
Sasaki Chloroquine potentiates the anti-cancer effect of 5-fluorouracil on colon cancer cells. BMC Cancer 2010 PMID: 20630104
Jiang Antitumor and antimetastatic activities of chloroquine diphosphate in a murine model of breast cancer. Biomed.Pharmacother. 2010 PMID: 20888174