CRID3 sodium salt

Name: CRID3 sodium salt
Brand: Tocris Bioscience
SKU: 5479

Tocris Bioscience, part of Bio-Techne | Catalog # 5479

Citations (17)

COA / SDS

Potent NLRP3 inflammasome inhibitor; inhibits IL-1β production

Key Product Details

Description:	Potent NLRP3 inflammasome inhibitor; inhibits IL-1β production
Alternative Names:	CP-456773,MCC 950
Chemical Name:	N-[[(1,2,3,5,6,7-Hexahydro-s-indacen-4-yl)amino]carbonyl]-4-(1-hydroxy-1-methylethyl)-2-furansulfonamide sodium salt
Purity:	≥98% (HPLC)
Molecular Weight:	426.46

View Full Technical Details for CRID3 sodium salt

Technical Data
Citations

Biological Activity for CRID3 sodium salt

CRID3 sodium salt is a potent NLRP3 inflammasome inhibitor; closes the active conformation of NLRP3 to the inactive state. Directly interacts with the Walker B motif within the NLRP3 NACHT domain. Inhibits IL-1β, IL-18 and IL-1α production (IC₅₀ values are 7.2, 10.3 and 12-18 nM, respectively). Selective for NLRP3 over NLRC4 inflammasome and Toll-like receptor signaling. Reduces severity of experimental autoimmune encephalomyelitis, skin inflammation and airway inflammation in mice. Also glutathione S-transferase omega 1 inhibitor. Orally bioavailable.

Licensing Information

Sold for research purposes under agreement from Pfizer Inc

Technical Data for CRID3 sodium salt

M. Wt	426.46
Formula	C₂₀H₂₃N₂NaO₅S
Storage	Store at +4°C
Purity	≥98% (HPLC)
CAS Number	256373-96-3
PubChem ID	91826093
InChi Key	LFQQNXFKPNZRFT-UHFFFAOYSA-M
Smiles	O=C(N(S(C4=CC(C(C)(O)C)=CO4)(=O)=O)[Na])NC1=C3C(CCC3)=CC2=C1CCC2

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for CRID3 sodium salt

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	water	42.65	100
	DMSO	42.65	100

Preparing Stock Solutions for CRID3 sodium salt

The following data is based on the product molecular weight 426.46.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.34 mL	11.72 mL	23.45 mL
5 mM	0.47 mL	2.34 mL	4.69 mL
10 mM	0.23 mL	1.17 mL	2.34 mL
50 mM	0.05 mL	0.23 mL	0.47 mL

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References for CRID3 sodium salt

References are publications that support the biological activity of the product.

Coll MCC950 directly targets the NLRP3 ATP-hydrolysis motif for inflammasome inhibition. Nat.Chem.Biol. 2019 PMID: 31086327
Tapia-Abellán MCC950 closes the active conformation of NLRP3 to an inactive state. Nat.Chem.Biol. 2019 PMID: 31086329
Coll A small-molecule inhibitor of the NLRP3 inflammasome for the treatment of inflammatory diseases. Nat.Med. 2015 PMID: 25686105
Gordon Inflammasome inhibition prevents a-synuclein pathology and dopaminergic neurodegeneration in mice. Sci.Transl.Med. 2018 PMID: 30381407
Laliberte Glutathione S-transferase omega 1-1 is a target of cytokine release inhibitory drugs and may be responsible for their effect on interleukin-1β posttranslational processing. J.Biol.Chem. 2003 PMID: 12624100