E 64

Name: E 64
Brand: Tocris Bioscience
SKU: 5208
Rating: 4 (1 reviews)

Tocris Bioscience, part of Bio-Techne | Catalog # 5208

(1)

Citations (10)

COA / SDS

Potent and irreversible cysteine protease inhibitor

Key Product Details

Description:	Potent and irreversible cysteine protease inhibitor
Chemical Name:	(2S,3S)-3-[[[(1S)-1-[[[4-(Aminoiminomethyl)amino]butyl]amino]carbonyl]-3-methylbutyl]amino]carbonyl]-2-oxiranecarboxylic acid
Purity:	≥98% (HPLC)
Molecular Weight:	357.41

View Full Technical Details for E 64

Technical Data
Citations / Reviews (1)

Biological Activity for E 64

E 64 is a potent and irreversible cysteine protease inhibitor (IC₅₀ values are 1.4, 2.5 and 4.1 nM for cathepsin K, L and S respectively. Also inhibits papain, calpain, cathepsin B and cathepsin H. Has no effect on serine proteases. Reduces HSC-3 tongue cancer cell invasion and induces oxidative stress and apoptosis in filarial parasites. Also inhibits autophagy-associated lysosomal degradation in vitro.

Technical Data for E 64

M. Wt	357.41
Formula	C₁₅H₂₇N₅O₅
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	66701-25-5
PubChem ID	123985
InChi Key	LTLYEAJONXGNFG-DCAQKATOSA-N
Smiles	O=[C@@](N[C@H]([C@](NCCCCNC(N)=N)=O)CC(C)C)[C@@H]1[C@@H](C(O)=O)O1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for E 64

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	water	7.15	20

Preparing Stock Solutions for E 64

The following data is based on the product molecular weight 357.41.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.2 mM	13.99 mL	69.95 mL	139.90 mL
1 mM	2.80 mL	13.99 mL	27.98 mL
2 mM	1.40 mL	6.99 mL	13.99 mL
10 mM	0.28 mL	1.40 mL	2.80 mL

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References for E 64

References are publications that support the biological activity of the product.

Zhao Cytosolic FoxO1 is essential for the induction of autophagy and tumour suppressor activity. Nat.Cell.Biol. 2010 PMID: 20543840
Bitu Cathepsin K is present in invasive oral tongue squamous cell carcinoma in vivo and in vitro. PLoS ONE 2013 PMID: 23951042
Wadhawan Inhibition of cathepsin B by E-64 induces oxidative stress and apoptosis in filarial parasite. PLoS ONE 2014 PMID: 24667798
Susa Human primary osteoclasts: in vitro generation and applications as pharmacological and clinical assay. J.Transl.Med. 2004 PMID: 15025786