GNF 7

Name: GNF 7
Brand: Tocris Bioscience
SKU: 5607

Tocris Bioscience, part of Bio-Techne | Catalog # 5607

COA / SDS

Ras signaling inhibitor; inhibits Ack1 and GCK

Key Product Details

Description:	Ras signaling inhibitor; inhibits Ack1 and GCK
Chemical Name:	N-[3-[1,4-Dihydro-1-methyl-7-[(6-methyl-3-pyridinyl)amino]-2-oxopyrimido[4,5-d]pyrimidin-3(2H)-yl]-4-methylphenyl]-3-(trifluoromethyl)benzamide
Purity:	≥98% (HPLC)
Molecular Weight:	547.53

View Full Technical Details for GNF 7

Technical Data
Citations

Biological Activity for GNF 7

GNF 7 is a potent Bcr-Abl inhibitor (IC₅₀ values are 61 and 133 nM for T315I and wild-type Bcr-Abl, respectively), inhibiting a range of clinically relevant Bcr-Abl mutants such as T315I, G250E, E255V, F317L and M351T. GNF 7 is also a Ras signaling inhibitor as well as inhibiting Ack1 and germinal center kinase (GCK). GNF 7 induces cell cycle arrest and apoptosis in leukemia cells harboring NRAS mutations, and shows growth inhibitory activity in human colon cancer cells. It prolongs survival in a leukemia cell xenotransplantation model in mice and is orally bioavailable.

Technical Data for GNF 7

M. Wt	547.53
Formula	C₂₈H₂₄F₃N₇O₂
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	839706-07-9
PubChem ID	11478363
InChi Key	SZNYUUZOQHNEKB-UHFFFAOYSA-N
Smiles	CC1=NC=C(NC2=NC=C(CN(C3=C(C)C=CC(NC(C4=CC(C(F)(F)F)=CC=C4)=O)=C3)C(N5C)=O)C5=N2)C=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for GNF 7

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	27.38	50

Preparing Stock Solutions for GNF 7

The following data is based on the product molecular weight 547.53.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.5 mM	3.65 mL	18.26 mL	36.53 mL
2.5 mM	0.73 mL	3.65 mL	7.31 mL
5 mM	0.37 mL	1.83 mL	3.65 mL
25 mM	0.07 mL	0.37 mL	0.73 mL

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References for GNF 7

References are publications that support the biological activity of the product.

Choi A type-II kinase inhibitor capable of inhibiting the T315I "gatekeeper" mutant of Bcr-Abl. J.Med.Chem 2010 PMID: 20604564
Nonami Identification of novel therapeutic targets in acute leukemias with NRAS mutations using a pharmacologic approach. Blood 2015 PMID: 25833960