Hh-Ag1.5

Name: Hh-Ag1.5
Brand: Tocris Bioscience
SKU: 7807

Tocris Bioscience, part of Bio-Techne | Catalog # 7807

COA / SDS

Potent and high affinity Smo receptor agonist

Key Product Details

Description:	Potent and high affinity Smo receptor agonist
Chemical Name:	3-Chloro-4,7-difluoro-N-[trans-4-(methylamino)cyclohexyl]-N-[[3-(4-pyridinyl)phenyl]methyl]benzo[b]thiophene-2-carboxamide
Purity:	≥98% (HPLC)
Molecular Weight:	526.04

View Full Technical Details for Hh-Ag1.5

Technical Data
Citations

Biological Activity for Hh-Ag1.5

Hh-Ag1.5 is a potent and high affinity Smoothened (Smo) receptor agonist (EC₅₀ = 1 nM; K_ivalues are 0.5 and 2.3 nM respectively, in a [³H]SAG-1.3 and [³H]Cyclopamine binding assay). It is used in protocols for the chemical reprogramming of mouse embryonic fibroblasts into neural stem cells. Also induces differentiation of hiPSCs into skin-derived precursor cells, spinal motor neurons and spinal sensory neurons.

Technical Data for Hh-Ag1.5

M. Wt	526.04
Formula	C₂₈H₂₆ClF₂N₃OS
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	612542-14-0
PubChem ID	44195701
InChi Key	HRUSRVLDSXRNFP-UPDYAAQLSA-N
Smiles	O=C(N(CC1=CC(C2=CC=NC=C2)=CC=C1)[C@H]3CC[C@H](NC)CC3)C(SC4=C(C=CC(F)=C54)F)=C5Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Hh-Ag1.5

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	DMSO	52.6	100
	ethanol	26.3	50

Preparing Stock Solutions for Hh-Ag1.5

The following data is based on the product molecular weight 526.04.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.90 mL	9.50 mL	19.01 mL
5 mM	0.38 mL	1.90 mL	3.80 mL
10 mM	0.19 mL	0.95 mL	1.90 mL
50 mM	0.04 mL	0.19 mL	0.38 mL

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References for Hh-Ag1.5

References are publications that support the biological activity of the product.

Sugiyama-Nakagiri Induction of skin-derived precursor cells from human induced pluripotent stem cells. PLoS One 2016 PMID: 27992514
Rominger Evidence for allosteric interactions of antagonist binding to the smoothened receptor. J.Pharmacol.Exp.Ther. 2009 PMID: 19304771
Takeda Chemical compound-based direct reprogramming for future clinical applications. Biosci.Rep. 2018 PMID: 29739872
Frank-Kamenetsky Small-molecule modulators of Hedgehog signaling: identification and characterization of Smoothened agonists and antagonists. J.Biol. 2002 PMID: 12437772