Skip to main content

MALP-2, TLR6 and TLR2 ligand

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-26219

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
Loading...
NBP2-26219-2ug
NBP2-26219

Key Product Details

Species

Non-species specific

Applications

Functional Assay

Product Summary for MALP-2, TLR6 and TLR2 ligand

Disclaimer Note -Disclaimer Note - Human Serum- No test method can provide total assurance that the hepatitis B virus, hepatitis C virus, human immunodeficiency virus, or any other infectious agents are absent. Thus, all blood products, including purified proteins derived from human blood sources, should be handled at Biosafety Level 2 as recommended by the CDC\NIH manual entitled Biosafety in Microbiological and Biomedical Laboratories for potentially infectious human serum, blood specimens or proteins derived from same. Source material for the human blood product supplied to your facility has been tested for the detection of HIV antibody, Hepatitis B surface antigen, antibody to Hepatitis C, HIV 1 antigen(s), antibody to HTLV - I/II, and syphilis by FDA guidelines. All units were found to be non-reactive/negative for these tests. All human blood source material is collected in FDA licensed centers and is tested with FDA approved test kits. Human Serum- No test method can provide total assurance that the hepatitis B virus, hepatitis C virus, human immunodeficiency virus, or any other infectious agents are absent. Thus, all blood products, including purified proteins derived from human blood sources, should be handled at Biosafety Level 2 as recommended by the CDC\NIH manual entitled Biosafety in Microbiological and Biomedical Laboratories for potentially infectious human serum, blood specimens or proteins derived from same. Source material for the human blood product supplied to your facility has been tested for the detection of HIV antibody, Hepatitis B surface antigen, antibody to Hepatitis C, HIV 1 antigen(s), antibody to HTLV - I/II, and syphilis by FDA guidelines. All units were found to be non-reactive/negative for these tests. All human blood source material is collected in FDA licensed centers and is tested with FDA approved test kits.

Product Specifications

Specificity

MALP-2 was originally isolated from Mycoplasma fermentans. This MALP-2 corresponds to the originally isolated isomer, which expresses potent endotoxin-like activity and approaches in certain experimental systems the toxicity of LPS. For description of the stereochemistry of MALP-2 please refer to M. Morr, et al. Eur. J. Immunol. 32, 3337 (2002). The importance of the stereochemistry of the central carbon atom of the diacylglycerol group has been described in the K.M. Omueti, et al. paper (2005), see below. Formula: C99H167N19O30S

Details of Functionality

Specific Activity is approx 2x10^8units/mg. One unit is defined as the dilution giving half maximal release of nitric oxide from C3H/HeJ mouse peritoneal exudate cells in the standard assay.

Application Notes

Source: Synthetic
Formula: C99H167N19O30S
MW: 2135.2
Use in In-vitro and functional assay reported in scientific literature (PMID: 25466255).

Scientific Data Images for MALP-2, TLR6 and TLR2 ligand

MALP-2, TLR6 and TLR2 ligand [NBP2-26219] - 293 cells were transfected with pCMV/TLR2-6 plasmid and pNF-kB/SEAP plasmid using Lipofectamin 2000. After 48 hrs of transfection, 50 and 100 ng/mL of MALP2 as added. Cells were incubated at 37C for 24 hrs. Transfected cell supernatant was collected and analyzed using the NF-kB SEAPorter Assay kit.
MALP-2, TLR6 and TLR2 ligand [NBP2-26219] - Evaluation of the MALP2 ligand activity on TLR2/NF-kB SEAPorter HEK 293 cell line.Cell line is a stably co-transfected cell line that expresses full-length human Toll-like receptor 2 (TLR2) and the secreted alkaline phosphatase (SEAP) reporter gene under the transcriptional control of an NF-kB response element. Cells were plated in 96-well plates at 5 x 10^4 cells/well. After 16 h, cells were stimulated with various amounts of MALP2 for 24 h. SEAP was analyzed using the SEAPorter Assay Kit. Data Summary: MALP2 specifically activated the TLR2/6-depedent NF-kB/SEAP reporter cells in a dose dependent manner. Note: HEK 293 cells endogenously express TLR6.
MALP-2, TLR6 and TLR2 ligand [NBP2-26219] - Differentiated human macrophages were treated with or without MALP-2 (100 ng/mL), and the release of IL-6 in culture supernatants of cells was measured by ELISA kit. Image submitted by a verified customer review.

Formulation, Preparation, and Storage

Formulation

2 ug in 20 uL of sterile PBS solution containing 2.5% (v/v) 2-propanol, 25 mM n-octyl-b-D-glucopyranoside and 1% (wt/v) human serum albumin.

Concentration

Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MALP-2, TLR6 and TLR2 ligand

MALP-2, TLR6 and TLR2 ligand sticks avidly to glass or plastic. Since it is active at very low concentrations, i.e. at high dilutions, these low amounts of material tend to become adsorbed to pipette tips and plastic or glass containers. To avoid this, prepare several dilution steps of the MALP-2, TLR6 and TLR2 ligand stock solution with medium containing 5% autologous serum or buffers with 2% HSA. The presence of 50mM octyl glucoside in the first dilution step is often beneficial and gives rise to higher activity. Avoid small volumes in large vessels. Do not filter.Because of the ester-bound fatty acids, MALP-2, TLR6 and TLR2 ligand is sensitive to alkali or acid. The medium should be preconditioned in the CO2 incubator in order to ensure that the pH does not become alkaline. Add cells in preconditioned medium as soon as possible and incubate.

Alternate Names

Macrophage-activating lipopeptide-2, MALP 2, MALP-2, TLR6 & TLR2 ligand, S-[2,3-bis(Palmityloxy)-(2R)-propyl-cysteinyl-GNNDESNISFKEK], TLR activator MALP2

Additional MALP-2, TLR6 and TLR2 ligand Products

Product Documents for MALP-2, TLR6 and TLR2 ligand

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for MALP-2, TLR6 and TLR2 ligand

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

Loading...
Loading...
Loading...