ML 210

Name: ML 210
Brand: Tocris Bioscience
SKU: 6429

Tocris Bioscience, part of Bio-Techne | Catalog # 6429

Citations (3)

COA / SDS

Selectively kills mutant HRAS-expressing cells; glutathione peroxidase inhibitor; induces ferroptosis

Key Product Details

Description:	Selectively kills mutant HRAS-expressing cells; glutathione peroxidase inhibitor; induces ferroptosis
Chemical Name:	[4-[Bis(4-chlorophenyl)methyl]-1-piperazinyl](5-methyl-4-nitro-3-isoxazolyl)methanone
Purity:	≥98% (HPLC)
Molecular Weight:	475.32

View Full Technical Details for ML 210

Technical Data
Citations

Biological Activity for ML 210

ML 210 is a glutathione peroxidase (GPX4) inhibitor. Kills mutant RAS-expressing cell lines (IC₅₀ values are 71 and 272 nM for HRAS G12v mutant expressing cell lines BJeLR and BJeH-LT, respectively). Exhibits 4-fold selectivity for HRAS mutant-expressing cell lines. Induces ferroptosis in tumor initiation "persister" cells and drug-resistant tumor cells.

Compound Libraries for ML 210

ML 210 is also offered as part of the Tocriscreen 2.0 Max. Find out more about compound libraries available from Tocris.

Technical Data for ML 210

M. Wt	475.32
Formula	C₂₂H₂₀Cl₂N₄O₄
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	1360705-96-9
PubChem ID	49766530
InChi Key	VIBHJPDPEVVDTB-UHFFFAOYSA-N
Smiles	CC1=C([N+]([O-])=O)C(C(N2CCN(C(C3=CC=C(C=C3)Cl)C4=CC=C(C=C4)Cl)CC2)=O)=NO1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for ML 210

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	23.77	50

Preparing Stock Solutions for ML 210

The following data is based on the product molecular weight 475.32.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.5 mM	4.21 mL	21.04 mL	42.08 mL
2.5 mM	0.84 mL	4.21 mL	8.42 mL
5 mM	0.42 mL	2.10 mL	4.21 mL
25 mM	0.08 mL	0.42 mL	0.84 mL

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References for ML 210

References are publications that support the biological activity of the product.

Yang Regulation of ferroptotic cancer cell death by GPX4. Cell 2014 PMID: 24439385
Hangauer Drug-tolerant persister cancer cells are vulnerable to GPX4 inhibition. Nature 2017 PMID: 29088702
Weïwer Development of small-molecule probes that selectively kill cells induced to express mutant RAS. Bioorg.Med.Chem.Lett. 2012 PMID: 22297109