MyD88 Inhibitor Peptide Set

Human, Mouse, Rat, Bovine, Xenopus, Yeast, Zebrafish

Block/Neutralize, ELISA, Flow Cytometry, Functional Assay, Immunohistochemistry, Inhibition Activity

The MyD88 Homodimerization inhibitory peptide contains a protein transduction (PTD) sequence (DRQIKIWFQNRRMKWKK) derived from antennapedia which renders the peptide cell permeable (Derossi et al, The third helix of the antennapedia homeodomain translocates through biological membranes. J Biol Chem. 269:10444-10450 (1994)] .The control peptide consists of only the PTD sequence.

Binding Inhibition (reported in scientific literature (PMID 28302163))
Block/Neutralize (reported in scientific literature (PMID 24886588))
ELISA (1:100 - 1:2000. Use reported in scientific literature (PMID 23469306))
Flow Cytometry (reported in scientific literature (PMID 27870876))
Functional (Inhibition) (reported in multiple pieces of scientific literature)
Immunohistochemistry (reported in scientific literature (PMID 28282921))
In vitro assay (reported in scientific literature (PMID 25810567))
In vivo assay (reported in scientific literature (PMID 24755282))

Inhibition of MyD88 dependent TLR/IL-1R signaling activitiy by interefering with MyD88 homodimer formation.

Inhibitory peptide at 100 uM concentration may be a starting point. However, useful concentration of peptide may vary depending on experimental condition and cell type. Incubate cells for 24 hr with peptides before stimulating with ligands.

Yeast reactivity reported in scientific literature (PMID: 27870876).

2 x 1.0mg: MyD88 Homodimerization Inhibitor peptide (lyophilized) DRQIKIWFQNRRMKWKKRDVLPGT (MyD88 homodimerization sequence: RDVLPGT). Molecular weight: 3100
2 x 1.0mg: Antennapedia Control peptide (lyophilized) DRQIKIWFQNRRMKWKK. Molecular weight: 2361
5mg size will contain 5mg of each peptide (MyD88 Homodimerization Inhibitor peptide and Antennapedia Control peptide).

Preparation of 5 mM Stock Solutions
PBS* is added directly to the vials to prepare the stock solutions. Note: Bring the solution to room temperature and quick spin the tubes before opening the caps.
MyD88 Homodimerization Inhibitor Peptide: 1 mg of DRQIKIWFQNRRMKWKKRDVLPGT
Add 64.4 ul of PBS* to the vial to make a 5 mM stock solution. Mix by vortexing. Aliquot and store at -20C or -80C. Avoid repeated freeze thawing.

Control Peptide: 1 mg of DRQIKIWFQNRRMKWKK
Add 84.8 ul PBS* to the vial. Mix by vortexing. Aliquot and store at -20C or -80C. Avoid repeated freeze thawing
*Recipe for 1X PBS:
1. Dissolve the following in 800ml distilled H2O.
- 8g of NaCl
- 0.2g of KCl
- 1.44g of Na2HPO4
- 0.24g of KH2PO4
2. Adjust pH to 7.5 with HCl.
3. Adjust volume to 1L with additional distilled H2O.
4. Sterilize by autoclaving

Solubilize the peptides prior to use by making 5 mM PBS* stock solutions (please see Preparation of 5 mM Stock Solutions under Preparation Method).

Lyoph

Please contact technical support for detailed reconstitution instructions.

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.